Upregulation of kinesin family member 4A enhanced cell proliferation via activation of Akt signaling and predicted a poor prognosis in hepatocellular carcinoma

a KIF4A mRNA expression in liver cancer tissues is higher than that in normal tissues from Oncomine database. Dataset 1, Wurmbach Liver; Dataset 2, Roessler Liver 1; Dataset 3, Roessler Liver 2; Dataset 4, Mas Liver. Cell colour and the number above the cell in the lower panel indicate the best gene rank percentile for the analysis. Red, upregulated; blue, downregulated. b The mRNA levels of KIF4A from 78 patients were tested by quantitative PCR using paired t-test. c The protein levels of KIF4A in HCC tissues and matched non-cancerous tissues from 18 patients with HCC were determined by western blotting assay. N non-cancerous, C cancer. Fold change of KIF4A protein with respect to non-cancerous specimens was normalized to GAPDH. The quantification of western blotting is shown in (d). e KIF4A protein expressions in nine HCC cell lines (QGY-7703, BEL-7404, Hepa3B, MHCC-97L, Huh7, PLC/PRF/5, BEL-7405, SMMC-7721, SK-HEP-1), two hepatoblastoma cell lines (HepG2, HepG2.215), and two immortalized liver cell lines (THLE-2 and LO2) were examined by western blotting. f Quantification of KIF4A expressions in different cell lines is show