c-FLIP is a target of the E3 ligase deltex1 in gastric cancer

a–c DTX1 expression is lower while CBL and ITCH expressions are normal or elevated in gastric intestinal type adenocarcinoma. Expression of DTX1 (a), CBL (b), and ITCH (c) mRNA in human gastric cancer tissue versus normal43–45. All three databases contain patients presenting with gastric intestinal type adenocarcinoma. The numbers of patient samples in each database are indicated in Fig. 1a. *P？≤？0.05, **P？≤？0.01, ***P？≤？0.001 by Student’s t-test (gastric intestinal type adenocarcinoma vs normal). Data were taken from Oncomine (www.oncomine.org). d–f DTX1 expression (d) is mostly downregulated, whereas CBL (e) and ITCH (f) expressions are mostly increased in different human gastric cancer cell lines. The names and types of the human gastric cancer cell lines from Ooi et al.45 are indicated at right. g–j Higher DTX1 expression in a gastric cancer cluster with better relapse-free survival (RFS). Kaplan–Meier plots (g) of two gastric cancer clusters of different relapse-free survival (RFS)46. P？=？0.001 by the log-rank test. Analysis of DTX1 (h), CBL (i), and ITCH (j) expression between C1 and C2 clusters. ***P？≤？0.001 by Student’s t-test NS, not significant