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- 2019
A novel hydrolase with a pro-death activity from the protozoan parasite Leishmania majorDOI: 10.1038/s41420-019-0178-2 Keywords: Apoptosis, Parasitic infection Abstract: a Alignment of the LmjF.36.6540 protein sequence and the PDB ID 4zi5, which is the crystal structure of dienelactone hydrolase-like promiscuous2 phospotriesterase p91 from metagenomic libraries, as done with the software Phyre2 (www.sbg.bio.ic.ac.uk/phyre2). The red stars indicate the three catalytic amino acids: C130, H204 and D175. b Tertiary structure of LmjF.36.6540 done with the UCSF Chimera software from the structural model from Phyre2. The catalytic triad is indicated in green. c LmjF.36.6540 electrostatic potential visualization: red for negative, white for neutral and blue for positive potentials (UCSF Chimera software, coulombic surface coloring). The catalytic triad is circled in green, highlighting the negative potential of the surface near the triad. d Alignment of the amino-acid sequences of the LmjF.36.6540 orthologs in different Leishmania specie
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