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-  2019 

A six-inhibitor culture medium for improving na?ve-type pluripotency of porcine pluripotent stem cells

DOI: 10.1038/s41420-019-0184-4

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Abstract:

a Live cell images of H1 hESC and Lv-piPSC under F and NHSM conditions with bars, 200?μm and AP staining images in NHSM condition with bars, 5?mm. b AP staining images of LV-piPSC following removal of individual components from NHSM. AP staining was performed at Day 5 and Day 10 of culture under each condition with bar, 5?mm. c Live cell (bar, 500?μm) and AP staining (bar, 5?mm) images of Lv-piPSC under NHSM, NHSM without TGFB1 and NHSM without TGFB1, plus TGFB inhibitor (TGFBi) conditions. d AP staining images of Lv-piPSC under FL6i condition and FL6i in absence of respective components (bar, 500?μm

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