ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury

C57Bl/6 mice were subjected to either sham operation or UUO. Mice were sacrificed at the indicated time. a Immunofluorescence staining for IL-1β (red) and DAPI (blue) on kidney sections. Scar bar: 20？μm. b Kidney tissue lysates were subjected to immunoblot analysis for pro-IL-1β, cleaved IL-1β and LC3. β-actin was used as a loading control. c Quantitative analysis of the ratio of LC3-II and pro-IL-1β to β-actin. Data are means？±？SEM (n？=？6 per group); *P？<？0.05 vs. sha