Role of the P2X7 receptor in inflammation-mediated changes in the osteogenesis of periodontal ligament stem cells

a Quantitative analysis of P2X7R mRNA expression in PDLSCs in normal α-MEM culture medium, inflammatory culture medium (medium supplemented with TNF-α and IL-1β), osteo-inductive culture medium, and inflammatory osteo-inductive culture medium (osteo-inductive culture medium supplemented with TNF-α and IL-1β). b Western blotting of P2X7R in PDLSCs in normal α-MEM culture medium, inflammatory culture medium (medium supplemented with TNF-α and IL-1β), osteo-inductive culture medium, and inflammatory osteo-inductive culture medium (osteo-inductive culture medium supplemented with TNF-α and IL-1β). Data are presented as the mean？±？S.D. for n from 4 to 6; ns indicates no significant difference between the indicated columns; *p？<？0.05, **p？<？0.01, and ***p？<？0.001 indicate significant differences between the indicated column