Asymmetrical methyltransferase PRMT3 regulates human mesenchymal stem cell osteogenesis via miR-3648

a, b Expression profiles of PRMT3 (a) and RUNX2 (b) during hMSCs osteogenic differentiation, determined by qRT-PCR analysis. Data are shown as mean？±？SD; n？=？3 independent experiments; *: compared with day 0, P？<？0.05; **: compared with day 0, P？<？0.01 by Student’s t-tests. c Western blotting analysis of PRMT3 and RUNX2 protein levels during osteoblastic differentiation of hMSCs. GAPDH was used as an internal control. PM, proliferation medium; OM, osteogenic medium. d Western blotting analysis of dimethyl-asymmetric methylation level of H4R3 during osteogenic differentiation of hMSCs. H4 was used as an internal control. e Micro-CT image and H&E staining of OVX mice at 6 weeks. Scale bar for micro-CT image: 1？mm; scale bar for H&E staining: 200？μm. f qRT-PCR analysis of Prmt3 mRNA level (left), western blotting analysis of PRMT3 and RUNX2 protein levels (middle), and H4R3me2a (right) in BMMSCs of OVX mice at 6 weeks. Data are shown as mean？±？SD; n？=？3 independent experiments; **P？<？0.01 compared with SHAM group by Student’s t-tests. g Micro-CT image and H&E staining of OVX mice at 12 weeks. Scale bar for micro-CT image: 1？mm; scale bar for H&E staining: 200？μm. h qRT-PCR analysis of Prmt3 mRNA level (left), western blotting analysis of PRMT3 and RUNX2 protein levels (middle), and H4R3me2a (right) in BMMSCs of OVX mice at 12 weeks. Data are shown as mean？±？SD; n？=？3 independent experiments; **P？<？0.01 compared with SHAM group by Student’s t-test