MEG3-derived miR-493-5p overcomes the oncogenic feature of IGF2-miR-483 loss of imprinting in hepatic cancer cells

a Experimental design of miRNA epigenetic unmasking. The cartoon illustrates the strategy used to identify miRNAs epigenetically silenced in liver cancer cells. b Expression profiles of the 122 miRNAs rescued in HepG2 cells in response to 5-AZA demethylating treatment (2.5？μM). Cells were collected every 2 days from days 0 (D0) to 12 (D12), and RNAs were extracted for microarray analysis. The scale bar encodes the logarithm of relative miRNA expression levels. miRNAs that were expressed before epigenetic unmasking were excluded from the analysis. ({"type":"entrez-geo","attrs":{"text":"GSE123314","term_id":"123314"}}GSE123314) c List of the miRNAs exhibiting the most significant re-expression after epigenetic unmasking (top 15). miR-127-3p, miR-136-5p, miR-376-a-3p, miR-376c-3p, miR-377-3p, and miR-493-5p were found to be located on chromosome 14 and associated with the imprinted DLK1-MEG3 locu