Loss of PICH promotes chromosome instability and cell death in triple-negative breast cancer

a PICH immunohistochemical scores in breast cancer tumors and matched adjacent normal breast epithelium. P-value was determined by the Mann–Whitney U test; n？=？194. b Representative images from immunohistochemical staining of PICH in tumors from two cases of breast cancer. The boxed areas in the left images are magnified in the right images. Scale bars, 150？μm (left), 50？μm (right). c Box plot of PICH expression in breast cancer from patient subjects divided in the HR+HER2？, HER2+, and TNBC subgroups (four patients without complete information on receptor expression were excluded). Differences between groups were analyzed by using a two-tailed unpaired t-test. *P？<？0.05, **P？<？0.01; ns, not significant. d Representative images of PICH staining in the three groups described in (c). Scale bar, 50？μm. e, f Kaplan–Meier estimates of distal metastasis-free survival (e) and overall survival (f) of the patients with breast cancer (n？=？169, the survival information for 25 of the 194 subjects was unavailable). Comparison was made between the high PICH expression (score？≥？6) and low PICH expression (score？<？6) groups. Marks on graph lines represent censored samples. P-value refers to two-sided log-rank tes