P2Y12 regulates microglia activation and excitatory synaptic transmission in spinal lamina II neurons during neuropathic pain in rodents

a Representative immunoblots of P2Y12 protein expression. Total cell lysates from the ipsilateral spinal cord were used, and GAPDH was used as an internal control. b Densitometric analysis (six rats per group) of P2Y12 proteins normalized to the loading control. c Representative immunofluorescence images of P2Y12 (green) staining in the spinal dorsal horn after SNL surgery. d Quantitative analysis of the number of P2Y12-immunoreactive cells per 400-μm visual field length per section per rat in the spinal dorsal horn after SNL surgery. Values are normalized to the sham group. n？=？3 rats per group, with three sections per rat. Values are represented as mean？±？SEM. *p？<？0.05 vs. sham group, **p？<？0.01 vs. sham group, ***p？<？0.001 vs. sham grou