Architecture, substructures, and dynamic assembly of STRIPAK complexes in Hippo signaling

a Schematic representation of the domain organization of the core STRIPAK components. CC, coiled-coil domain; WD40, WD40 repeat; Kinase, kinase domain; SARAH, Sav-RASSF-Hpo domain; NTD, N-terminal domain; CTD, C-terminal domain; FHA, forkhead-associated domain; TM, transmembrane domain. b Effect of the knockdown of STRIPAK components on the activation of Hippo signaling in HGC-27 cells. n.c., negative control (control siRNA). c Real-time PCR analysis of the expression levels of CTGF in HGC-27 cells transfected with the indicated siRNAs. Bar graphs represent the means？±？SD. Experiments were repeated three times. Unpaired t tests were used to compare the difference between the two groups. *Significant relative to control, p？<？0.05, **p？<？0.01, ***p？<？0.001. d MBP pulldown analysis of the PP2A holoenzyme containing STRN3 as its B′′′ subunit. The input and output samples were loaded on an SDS-PAGE gel followed by staining this gel with Coomassie brilliant blue (CBB). e MBP pulldown analysis of the interactions between core STRIPAK components and the scaffold protein STRN3. The input and output samples were loaded on an SDS-PAGE gel followed by staining this gel with CBB. f MBP pulldown analysis of the interactions between core STRIPAK components and the MST2 protein kinase. The input and output samples were loaded on an SDS-PAGE gel followed by staining this gel with CBB. g Model of the overall assembly of the STRIPAK comple