The exon 19-deleted EGFR undergoes ubiquitylation-mediated endocytic degradation via dynamin activity-dependent and -independent mechanisms

EGF treatment leads to EGFR downregulation in NSCLC cells. Indicated NSCLC cells were serum-starved and treated with EGF at 20 ng/ml for different time periods. The levels of EGFR were detected by immunoblotting assays with an EGFR antibody. The intensities of EGFR bands were quantified and adjusted according to corresponding GAPDH levels, which were used to plot degradation curves (shown below blots). All error bars represent the standard error of the mean (n？=？3