Comparative transcriptomic analysis of hematopoietic system between human and mouse by Microwell-seq

a A gene expression heat map showing the differential gene expression for each cell cluster in human single hematopoietic stem and progenitor cell (HSPC) data. Rows correspond to individual genes found to be selectively upregulated in individual clusters; columns are individual cells, ordered by clusters. Yellow corresponds to high expression level; purple and black correspond to low expression level. b A gene expression heat map showing the differential gene expression for each cell cluster in mouse single HSPC data. The color scale and sample layout are the same as in Fig. 1a. c Fraction of cells in each human cluster was assigned to each mouse cluster based on orthologous genes. Red corresponds to high correlation level; white correspond to low correlation level. d t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis of human-mouse HSPC compartment cell data. Cells are colored by experimental samples (left). Cells are colored by cell type cluster (right). e A table showing the confirmation and gene symbols of 16 cell types. f A bar showing the proportion of human and mouse cells of all progenitors. g t-SNE analysis of human CD45+CD34+ cells. Human peripheral blood CD34+ cells were transplanted into sublethally irradiated NCG mice, and human CD45+CD34+ cells were sorted from mouse bone marrow 2 months after transplantation. Cells are colored by cell type cluster. Lineage-primed progenitors like pro-neutrophil, pro-megakaryocyte, pro-T, and pro-B in CD45+CD34+ cells were visualized on the t-SNE map. h A gene expression heat map showing the differential gene expression for each cell cluster in human CD45+CD34+ cell data. Yellow corresponds to high expression level; purple and black correspond to low expression level. i The differentiation process of neutrophils and neutrophil progenitors from mouse and human datasets were plotted on the two-dimension space by Monocle. j The gating scheme of defined neutrophil progenitors (CD114+/CD177+) and erythrocyte progenitors (CD71+) from MPP (CD34+CD38-Thy1-CD45RA-CD49f-) cells. MPP cells were sorted from mobilized peripheral blood (mPB), bone marrow (BM) cells by FACS. k The percentage of different colony types in the colony-forming assay from CD71+ and CD71？ MPP-mPB, CD114+ and CD114？ MPP (mPB and BM). The results showed the average percentage from three independent experiments. l Single-cell qPCR was used to detect the expression of erythrocyte and neutrophil markers in the cells from colony-forming assay. The y-axes mean percentage of single cells expressing erythrocyte markers from CD71+ and CD71？