EZH2, JMJD3, and UTX epigenetically regulate hepatic plasticity inducing retro-differentiation and proliferation of liver cells

a Total protein lysates were extracted from proliferating HepaRG (GM) and differentiated HepaRG cells for the indicated time. Cells were harvested and the immunoblotting analysis was performed using specific antibodies (Table S4). b Total RNAs were extracted from pHepaRG (GM) and dHepaRG (DM) cells, qPCR analysis was performed using specific primers (Table S3). Amplification of GAPDH transcripts was used to normalize equal loading of each RNA samples. Histograms show the fold induction of DM versus GM. c Nuclear acid protein lysates from pHepaRG (GM) and dHepaRG cells (DM) treated or not with GSK-J4 for 48？h were analyzed by Immunoblot (left panel) with the indicated antibodies (Table S4); right panel: densitometric analysis is expressed as fold induction (FI) of DM, DM？+？GSK-J4 versus GM cells. d Optical microscope images of HepaRG cells treated as in (a). e Total RNAs were extracted from dHepaRG (DM) cells treated or not with GSK-J4 for 48？h and qPCR analysis was performed using specific primers (Table S3). Amplification of GAPDH transcripts was used to normalize equal loading of each RNA samples. Histograms show the fold induction of treated cells (GSK-J4) versus untreated (DM). All results are expressed as fold induction (mean) from three independent experiments, bars indicate S.D.; Asterisks indicate P-value: *0.01？≤？P？<？0.05; **0.001？≤？P？<？0.01; ***P？<？0.00