AID modulates carcinogenesis network via DNA demethylation in bladder urothelial cell carcinoma

a–d AID was detected by western blot and IHC in human BUCC/para-BUCC tissues. Original magnification: c,？×？200, calibration bar at 50？μm. The expression of AID in human BUCC tissues was more than that in para-BUCC tissues (BC: 0.98？±？0.19, PC: 0.16？±？0.05, P？<？0.05). d, e The similar results found by western blot in BUCC cell lines; the relative expression of AID in bladder cancer cells T24 and 5637 was significantly more than that in SV-HUC-1 cell (T24: 0.73？±？0.07, 5637: 0.72？±？0.04, SV-HUC-1: 0.13？±？0.04, P？<？0.05). SV-HUC-1 was used as a negative control. All the experiments were repeated three time