Long noncoding RNA DIO3OS interacts with miR-122 to promote proliferation and invasion of pancreatic cancer cells through upregulating ALDOA

DIO3OS expression is up-regulated in human PC tissues and PC cell lines. a DIO3OS expression pattern was analyzed in a panel of cancer (red) vs. normal (green) tissues from the MethHC database. Pancreatic adenocarcinoma: PAAD. b Expression of DIO3OS in PC samples and normal pancreatic tissues. The Cancer Genome Atlas (TCGA) datasets were retrieved in the UALCAN web server. c qRT-PCR analysis of DIO3OS levels in four PC cell lines and normal pancreatic cell line HPDE6-C7. d Kaplan–Meier curves for overall survival of PC patients were compared between groups with high or low levels of DIO3OS. e Expression of miR-122 in two PC cell lines and normal pancreatic cell line HPDE6-C7 was examined using qRT-PCR analysis. f Western blot analysis of ALDOA expression in PC cell lines and normal cell line HPDE6-C7. The membranes were cut prior to exposure so that only the portion of gel containing desired bands would be visualized. g Expression of miR-122 in PC samples and normal pancreatic tissues. The Cancer Genome Atlas (TCGA) datasets were retrieved from the MethHC database. h The levels of ALDOA in PC samples and normal pancreatic tissues. The Cancer Genome Atlas (TCGA) datasets were retrieved using the UALCAN web server. *P？<？0.05. **P？<？0.00