Long non-coding RNA CASC2 upregulates PTEN to suppress pancreatic carcinoma cell metastasis by downregulating miR-21

CASC2 suppressed metastasis of the PANC-1 pancreatic carcinoma cell. a Levels of CASC2 expression are low in the pancreatic carcinoma cells. Expression of CASC2 in the human pancreatic cancer cell lines and normal pancreatic HPDE6-C7 cells was detected by qRT-PCR. **P？<？0.01 vs. HPDE6-C7. b–d CASC2 sequences were ligated into the pEX-2 vector (pEX-CASC2). An empty pEX-2 vector was used as a negative control (pEX). Pancreatic carcinoma cells were transfected with the CASC2-expressing vector (pEX-CASC2) or the corresponding negative control (pEX) for 48 h. The cells without transfection were used as a control (CT). b Expression of CASC2 in the cells. c Cell migration and d invasion were assessed by the transwell assay (n？=？3; 10 random fields were counted). ***P？<？0.001. Scale bar: 100 μ