Efficient production and secretion of oxaloacetate from Halomonas sp. KM-1 under aerobic conditions

Oxaloacetate, pyruvate, and bioplastic poly-(R)-3-hydroxybutyric acid production by Halomonas sp. KM-1 with different NaCl concentrations. KM-1 cells were cultured under aerobic conditions (agitation speed: 200 rpm) at 37 °C. The medium was composed of modified SOT medium, with the control (circles), extra 0.3 M (triangles), extra 0.8 M (squares), or extra 1.3 M (diamonds) NaCl samples shown. a Cell dry mass (CDM), b intracellular bioplastic poly-(R)-3-hydroxybutyric acid (PHB), c oxaloacetate, d pyruvate in the medium, and e glucose in the medium were analyzed. The data shown represent the mean ± SD of five independent experiment