Protective Effects of Hesperidin and Coenzyme Q10 on Experimental Gastrocnemius Muscle Ischemia/Reperfusion Model in Rats

Abstract In this study, we aimed to investigate the effects of hesperidin and coenzyme Q10 on muscle ischemia/reperfusion injury in a rat model. A total of 28 Sprague-Dawley male rats were divided into four groups: Control, Sham, I/R+CoQ10 and I/R+Hesperidin groups. All rats were anesthetized with xylazine and ketamine. Except for the control group, the left lower limbs of the other 3 groups were applied to 2 hours of ischemia and 2 hours of reperfusion with tourniquet. TNF-α, IL-6, GSH, MDA levels, CAT, GPx, SOD activities were measured in the plasma. The levels of MDA with XO, CAT, SOD activities were determined in tissues. Increases in TNF-α, IL-6 and MDA levels, decreases in the CAT, GPx and SOD activities were significant in the sham group according to the control group but the differences in GSH were insignificant in plasma samples. The decreases in plasma TNF-α, IL-6 and MDA levels, the increases in CAT, GPx and SOD activities were statistically significant in the experimental groups compared to the sham group, but the GSH differences were insignificant. MDA and XO levels were higher in the experimental groups than in the sham and control groups, CAT and SOD levels decreased, these differences were statistically significant. The results of this study support the possibility that hesperidin and CoQ10 may play a protective role against skeletal muscle injury caused by I/R in rats by reducing oxidative stress. Hence, hesperidin and coenzyme Q10 may be useful adjunctive therapeutic agents in some operations suspected of organ I/R damage