酸性环境抑制CIK细胞对肝癌HepG2细胞的杀伤活性
DOI: 10.3971/j.issn.1000-8578.2016.05.003
Keywords: CIK,酸性环境,HepG2,杀伤活性,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effects of Silencing B7-H3 Gene on Invasion of Human Hepatocellular Carcinoma Cells,Betanin Induces Nuclear Necrosis and Mitochondria Intact of HeLa, HepG2 and A549 Cells,Establishment of Hepatocellular Carcinoma Cell Line HepG2 Stably Transfected by Hepatitis B Virus X Gene and Its Effects on p53-p21 Pathway,Screening of Effective STAT5A siRNAs and Their Influence on Proliferation and#br# Apoptosis in Human Hepatocellular Carcinoma Cell Line HepG2,Effects of SEPT9 Gene Silencing on Cell Proliferation and Apoptosis of HepG2 Hepatoma Cells,Expressions of DR5,caspase-8,caspase-9 and caspase-3 in HepG2 Cell Line Treated with Gemcitabine,Effects of Intrathoracic DC-CIK Treatment and Chemotherapy on Immune Indexes in Malignant Pleural Effusion,Effects of 5-Aza-2′-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2,Inhibitory Effects on HepG2 Cells from Genetic Engineering Cells Secreted Tumor Necrosis Factor,Effect of CIK Treatment on Localized Renal Carcinoma Patients after Radical Operation,Significance of Caspase in NS-398 Induced Apoptosis of HepG2 Cell Line,Anti-proliferative Effect of Combining Paeonol and Doxorubicin on HepG2 Cell,Anti-proliferation and Inducing Apoptosis Effects of CIK Cells Against Breast Cancer Cell Lines,Cloning and Identification of Caspase-3 Gene from HepG2 Cells that Underwent Autophagic Apoptosis
Abstract:
摘要 目的 研究酸性环境对CIK（cytokine-induced killer, CIK）细胞杀伤肝癌HepG2细胞的影响。方法 利用IFN-γ、IL-2及CD3抗体诱导外周血单个核细胞获得CIK细胞。在pH6.5及pH7.4条件下将CIK细胞和荧光素酶标记的HepG2细胞（HepG2-luc）按不同的效靶比混合培养，用小动物活体成像系统检测HepG2-luc荧光强度并计算杀伤活性，用MTT法检测并计算杀伤活性。在pH6.5及pH7.4条件下，在含CIK条件培养液0、50%和100%的情况下培养HepG2细胞，流式细胞仪检测凋亡坏死的细胞比例。结果 pH7.4时CIK细胞对HepG2细胞的杀伤率明显高于pH6.5时。CIK条件培养液作用下，pH7.4时HepG2细胞的凋亡坏死比例明显高于pH6.5。结论 酸性环境明显抑制了CIK细胞对肝癌细胞HepG2的杀伤活性
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