组蛋白去乙酰化酶抑制剂对胶质瘤细胞增殖及MKK7表达的影响
DOI: 10.3971/j.issn.1000-8578.2016.01.001
Keywords: 神经胶质瘤,组蛋白去乙酰化酶抑制剂,MKK7,增殖,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP,Expression of Toll-like Receptor 4 in Lymphoma Cell Lines and Its Effect on Cells Proliferation and Drug Resistance,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells,Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO,Inhibitory Effects of IWR-1 on Hepatic Carcinoma Hep3B Cells Proliferation and Wnt/β-catenin Signaling Pathway,LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma,Effect of exosome Extracted from Fibroblast Cell Line 3T3 on Proliferation of Mouse Breast Cancer Cells,miRNA-26a Inhibits Proliferation and Invasion of Breast Cancer Cells Through Downregulating COX-2 Expression,SRC Regulates Glioma Proliferation, Migration, Invasion and Cancer Stem-like Cell Self-renewal
Abstract:
摘要 目的 探讨组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor, HDACI）对胶质瘤细胞增殖及MKK7表达的影响。方法 体外培养神经胶质瘤细胞株U251，用脂质体转染MKK7-siRNA1、MKK7-siRNA2和对照siRNA，48 h后Western blot检测MKK7表达及JNK/c-Jun活性，BrdU掺入实验检测细胞增殖情况；用0.5 μM组蛋白去乙酰化酶抑制剂曲古菌素A（trichostatin A, TSA）处理细胞8 h，DMSO作对照，Western blot检测MKK7、p-JNK、JNK、p-c-Jun、c-Jun表达或磷酸化水平；用TSA分别处理细胞8、12、24 h，检测各时间点细胞增殖率；用其他组蛋白去乙酰化酶抑制剂包括伏立诺他（suberoylanilide hydroxamic acid, SAHA），丙戊酸（valproic acid, VPA），M344处理细胞检测MKK7表达及细胞增殖。结果 同对照组相比，沉默MKK7表达抑制了JNK/c-Jun活性和细胞增殖（P=0.008）；TSA处理细胞8 h显著抑制MKK7表达及JNK/c-Jun活性，SAHA、M344、VPA均显著抑制MKK7表达。TSA处理细胞8 h没有抑制细胞增殖，处理12 h显著抑制增殖（P=0.006）， 24 h抑制效应更明显（P=0.002）； SAHA（P=0.001）, M344（P=0.008）或VPA（P=0.002）处理细胞12 h均抑制了细胞增殖。结论 组蛋白去乙酰化酶抑制剂可通过抑制MKK7表 达抑制神经胶质瘤细胞增殖
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