miR-198靶向MAPK1调控宫颈癌HeLa细胞增殖、凋亡和侵袭
DOI: 10.3971/j.issn.1000-8578.2018.18.0537
Keywords: 宫颈癌,miR-198,MAPK1,凋亡,侵袭,Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells
Abstract:
摘要 目的 探究miR-198对宫颈癌细胞增殖、凋亡和侵袭的作用及其机制。方法 用miR-198 mimic转染HeLa细胞，qRT-PCR检测miR-198和丝裂原活化蛋白激酶1 （Mitogen-activated protein kinase1, MAPK1）的mRNA水平；荧光素酶实验检测miR-198和MAPK1的靶向关系；用pcDNA3.0-MAPK1（pc-MAPK1）和miR-198 mimic转染细胞，CCK-8检测细胞增殖活性，流式细胞术检测细胞凋亡，Transwell法检测细胞侵袭能力，Western blot检测蛋白的表达。结果 miR-198 mimic转染细胞后，miR-198表达水平明显升高，MAPK1 mRNA表达水平明显降低；荧光素酶报告实验表明miR-198序列上存在MAPK1结合位点；miR-198过表达能显著降低宫颈癌细胞增殖倍数、诱导细胞凋亡，同时还能明显降低HeLa细胞侵袭能力；此外，miR-198 mimic能显著抑制MAPK1下游基因核糖体S6激酶2（Ribosomal S6 kinase 2, RSK2）、c-Myc和c-fos的蛋白表达；pc-MAPK1能明显减弱miR-198 mimic对HeLa细胞增殖、凋亡、侵袭及对MAPK1下游蛋白表达的调控作用。结论 miR-198过表达能通过靶向MAPK1抑制宫颈癌细胞的增殖和侵袭能力并诱导细胞凋亡
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