紫杉醇诱导的卵巢癌耐药细胞株的生物学特性及其保存
DOI: 10.3971/j.issn.1000-8578.2015.04.004
Keywords: 紫杉醇,耐药,卵巢癌,Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma,Effect of Cisplatin Combined with Buzhong Yiqi Decoction on survivin Expression in Transplantation Tumor of Nude Mice with A549/DDP Cells,Experimental Investigation on Reversing Drug Resistance of Non-small Cell Lung Cancer Stem Cells by Aqueous Extract of Taxus Chinensis,Effect of CGB5 on Human Epithelial Ovarian Cancer Cell Line OVCAR-3 in Vasculogenic Mimicry Formation,UGCG is Involved in Oxaliplatin Resistance Mechanism of Human Colon Cancer Through Regulating MDR1/P-gp Expression,Experiment of Different Administration Sequences of Apatinib and Paclitaxel on Lung Cancer,Predictive Factors for Customizing Chemotherapy on Advanced Lung Adenocarcinoma,Bioinformatics Analysis of Genes Related to Multidrug Resistance in Ovarian Cancer,Expression of Toll-like Receptor 4 in Lymphoma Cell Lines and Its Effect on Cells Proliferation and Drug Resistance,Knockdown of PKD1 Decreased Sensitivity of Human Salivary Gland Adenoid Cystic Carcinoma Cell Line ACC2 to Paclitaxel,Efficacy and Safety of Weekly Liposome-paclitaxel or Oxaliplatin Combined with Tegafur on Elderly Advanced Gastric Cancer,Impact of miRNA-200c on Methotrexate Resistance of Non-small Cell Lung Cancer Cells A549,Mechanism of Jinlong Capsule Reversing Paclitaxel Resistance of A549 Cells Investigated by System Biology Technology,miR-143/145 Cluster Regulate Multi-drug Resistance of Small Cell Lung Cancer,Clinical Investigation on Cytoreductive Surgery plus Hyperthermic Intraperitoneal Chemotherapy on Patients with Peritoneal Carcinomatosis from Epithelial Ovarian Cancer
Abstract:
摘要 目的 研究紫杉醇诱导的卵巢癌耐药细胞株的生物学特性，探索耐药细胞的长期保存条件，以建立稳定的体外实验模型。方法 选用卵巢癌化疗敏感细胞株SKOV3，分别采用大剂量冲击和小剂量浓度递增诱导建立耐药的卵巢癌细胞株SKOV3/TAX300、SKOV3/TAX30。四甲基偶氮唑盐（MTT）法检测耐药细胞对化疗药物紫杉醇的IC50；比较耐药细胞和敏感细胞的细胞平板克隆形成、生长曲线倍增时间的差异；荧光定量PCR检测敏感细胞和耐药细胞中耐药相关基因的表达；比较无药冻存和加药冻存的方式对耐药细胞的保存效果。结果 耐药细胞SKOV3/TAX300，耐药指数为4.60，耐药细胞SKOV3/TAX30，耐药指数为51.31；与敏感细胞相比，耐药细胞体积增大，形态不规则，倍增时间延长。多药耐药基因1（MDR1）在两种耐药细胞中高表达，肺耐药相关蛋白（LRP）、蛋白激酶Cα（PRKCA）基因仅在SKOV3/TAX300中的表达增高，BCL-2样1基因（BCL2L1）在SKOV3/TAX30中低表达。耐药细胞冻存在含有药物的条件下，有利于耐药性的保持。结论 大剂量冲击和小剂量浓度递增两种方法诱导的耐药细胞为研究紫杉醇耐药机制建立了良好的体外模型，两种耐药细胞SKOV3/TAX300和SKOV3/TAX30具有不同的生物学特性
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