碘125粒子对乳腺癌细胞VEGF表达的抑制作用
DOI: 10.3971/j.issn.1000-8578.2015.02.003
Keywords: 125I粒子,乳腺癌,血管内皮细胞生长因子,Multi-factors Affect Cardiac Toxicity in Radiotherapy on Left-sided Breast Cancer,Effect of Neoadjuvant Chemotherapy Cycles on Pathologic Complete Response Rate Under Different Estrogen Receptor Status of Breast Cancer,Review of Targeted Therapy for Breast Cancer with BRCA Genes Mutation,Autophagic Cell Death Induced by GANT61 Inhibits Proliferation of Human Breast Cancer Cell Line MCF-7,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Progress on FOXP3 Expression in Breast Cancer Cells,Effect of Hormones from Adrenal Gland on Breast Cancer Metastasis in Mice,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Analysis E-cad and VEGF Expression in Triple-negative Breast Cancer of Han and Uygur,Regulations and Roles of Breast Cancer Stem Cells,Prediction of Disease Specific Survival Rates by Genes Associated with Relapse of Breast Cancer,Correlation Between Tumor Associated Macrophages and CXCL12 Expression in Different Breast Tissues,Effects of NRP-1 Overexpression on Biological Behaviors of Breast Cancer Cell Lines MDA-MB-231 and SK-BR-3,Effect of Operation Incision Selection on Success rate of Sentinel Lymph Node Biopsy and Subcutaneous Hydrops in Breast Cancer,Expression and Clinical Significance of Artemin Protein in Triple-negative and Nontriple-negative Breast Cancer Tissues
Abstract:
摘要 目的 探讨125I粒子对乳腺癌血管内皮细胞生长因子(VEGF)表达的影响及其抑制肿瘤细胞增殖的作用机制。方法 建立人类乳腺癌细胞株MCF-7裸鼠皮下移植瘤模型，随机分为8组，对照组为C7、C14、C21、C28组，实验组为T7、T14、T21、T28组，每组6只，对照组植入空白粒子（0Bq），实验组植入125I粒子（14.8MBq）。观察粒子植入后瘤体生长情况，分别在粒子植入后7、14、21、28 d四个时间点处死两组荷瘤裸小鼠。用半定量RTPCR、免疫组织化学染色方法检测VEGF基因及其蛋白的表达。结果 植入125I粒子后，实验组肿瘤生长缓慢，肿瘤抑制率为53.27%。粒子植入28 d，与对照组相比，实验组中肿瘤组织的VEGF mRNA及蛋白表达明显降低，差异有统计学意义。结论 125I粒子抑制乳腺癌细胞增殖重要分子生物学机制之一是其抑制乳腺癌细胞VEGF基因和蛋白的表达
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