抑癌基因TCF21启动子甲基化在肾癌诊断及预后中的意义
DOI: 10.3971/j.issn.1000-8578.2016.05.012
Keywords: Partial Versus Radical Nephrectomy,Correlation of ChREBP mRNA Expression and Its CpG Island Methylation in Human Hepatocellular Carcinoma,Progress of Epigenetic Regulation of H3K27me3 and EZH2 in B-cell Non-Hodgkin's Lymphoma,Progress of DNA Methylation in Minimal Residual Disease Detection and Related#br# Therapy for Leukemia,Effects of Folate with Different Oxidative States on Genomic DNA Methylation in#br# Human Lymphoblast Cell Line,Progress and Development Trend of Treatment for Urological Tumor,Methylation of Tumor Suppressor Gene TIP30 in Promoter Region for Colorectal Cancer Diagnosis and Prognosis,Meta-analysis on Relationship between runx3 Gene and Hepatocellular Carcinoma,Clinical Efficacy of Autologous CIK Cell Adoptive Immunotherapy Combined with Chemotherapy in Patients Withmetastatic Renal Cell Carcinoma,Methylation State of WWOX Gene Promoter CpG Islands in Epithelial Ovarian Cancer and Its Clinical Significance,Effect of TIP30 on Biological Characters of HCT116 Cells,Application of 64-slice CT Imaging in Laparoscopic Radical Nephrectomy
Abstract:
摘要 目的 探讨TCF21启动子甲基化在肾癌诊断与预后中的意义。方法 使用焦磷酸测序仪对样本的TCF21基因启动子甲基化水平进行定量检测，并进行统计学分析。结果 肾癌组织及尿液中TCF21基因甲基化水平明显升高（P=0.000,P=0.000）。肾癌组织TCF21基因甲基化水平与年龄（P=0.003）、吸烟史（P=0.018）、Fuhrman分级（P=0.046）及临床分期（P=0.048）有关， TCF 2 1 甲基化水平与年龄（ r=0 . 4 0 3 、P=0 . 0 0 2 ） 、吸烟史（r=0.321、P=0.017）和Fuhrman分级（r=0271、P=0.045）呈正相关。肾癌尿液中TCF21甲基化水平与肿瘤大小（P=0.000）、Fuhrman分级（P=0.013）及临床分期（P=0.020）有关，TCF21甲基化水平与肿瘤大小（r=0.662、P=0.000）、Fuhrman分级（r=0.662、P=0.010）和临床分期（r=0.662、P=0.017）呈正相关。TCF21高甲基化组（>35.42%）与TCF21低甲基化组（≤35.42%）患者术后生存率和总生存率差异有统计学意义（P=0.000，P=0.000）。肾癌组织中TCF21甲基化水平诊断肾癌的ROC曲线下面积高于尿液样本，差异具有统计学意义（P=0.004）。结论 TCF21与肾细胞癌的发生、发展及预后有关。联合检测肾细胞癌组织标本和尿液标本可作为诊断肾癌及判断预后等生物学行为的重要指标
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