PTTG1促进结肠癌SW480细胞侵袭和迁移的作用机制
DOI: 10.3971/j.issn.1000-8578.2016.11.006
Keywords: A Comparison Between Immunohistochemistry and PCR Method,NS-398 Reverses Multi-drug Resistance of Colorectal Carcinoma Cells,Plasma miR-21 Expression in Colon Cancer Patients Before and After Operation and Its Clinical Significance,Effects of Aspirin on VEGF, β-catenin Expression in Colon Cancer Cell Lines SW480 and HT-29,Effect of Oxaliplatin on CD44V6, VEGF, survivin, Caspase-3 and Caspase-7 in Dimethylhydrazine-induced Colon Cancer Rats,Screening of Long Non-coding RNA in Colon Cancer by Bioinformatics,Expression of Leptin and Leptin Receptor in Colon Cancer and Impact of Exogenous Leptin on Proliferation and Apoptosis of Colon Cancer Cell Line HT-29,Relationship Between Preoperative Fibrinogen Level and Clinicopathological#br# Characteristics of Colon Cancer
Abstract:
摘要 目的 研究垂体肿瘤转化基因 1（pituitary tumor transforming gene 1, PTTG1）过表达促进人结肠癌细胞SW480侵袭和迁移作用及其可能机制。方法 采用脂质体转染法将pcDNA3.1(+)-PTTG1及空载pcDNA3.1(+)转染人结肠癌SW480细胞，G418法筛选阳性克隆。Western blot和Real-time PCR法鉴定稳定过表达PTTG1细胞株建立。Transwell小室法检测细胞侵袭和迁移能力，Western blot检测MMP2、MMP9、E-cadherin、Vimentin和Snail的表达。结果 （1）成功获得稳定高表达PTTG1的SW480克隆细胞株PTTG1-SW480；（2）过表达PTTG1基因后，SW480细胞侵袭和迁移能力增强，MMP2和MMP9表达升高，上皮间质转化（epithelial-mesenchymal transition, EMT）标记分子E-cadherin表达降低，Vimentin和Snail的表达升高，差异均有统计学意义（P<0.01）；（3）过表达PTTG1基因后，SW480细胞中PI3K/AKT信号活化增强，使用LY29400干预后，抑制细胞侵袭、迁移和EMT，E-cadherin表达上调、Vimentin和Snail的表达下调，差异均有统计学意义（P<0.01）。结论 PTTG1基因过表达可能通过活化PI3K/AKT信号诱导SW480细胞EMT发生，发挥促进SW480侵袭和迁移作用；提示PTTG1蛋白可能成为结肠癌治疗的一个潜在靶点
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