敲低膜联蛋白A5对人胃癌细胞系MKN-45增殖的作用
DOI: 10.3971/j.issn.1000-8578.2018.18.0396
Keywords: 胃癌,膜联蛋白A5,MKN-45细胞,增殖,凋亡,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
Abstract:
摘要 目的 探讨膜联蛋白A5低表达对人胃癌MKN-45细胞增殖和凋亡的影响。方法 将胃癌MKN-45细胞分为siRNA干扰组、阴性对照组和空白对照组。采用RNA干扰技术将靶向膜联蛋白A5的siRNA和阴性对照siRNA脂质体转染法转染细胞，转染48 h后采用qRT-PCR和Western blot法分别在mRNA水平和蛋白水平进行抑制效率的鉴定，空白对照组不予任何处理。应用MTT、平板克隆形成法检测膜联蛋白A5低表达对胃癌MKN-45细胞增殖的影响，流式细胞术检测细胞凋亡情况。Western blot法检测增殖核抗原PCNA和周期相关蛋白Cyclin D1的表达。结果 靶向膜联蛋白A5的siRNA转染后，胃癌MKN-45细胞中膜联蛋白A5的表达较阴性对照和空白对照组明显降低（P<0.05）。MTT和克隆形成实验可见siRNA干扰组细胞增殖活力和集落形成能力较阴性对照和空白对照组显著增高（P<0.05）；流式细胞术显示细胞凋亡率无明显改变；PCNA和Cyclin D1的表达显著上调。结论 膜联蛋白A5低表达能促进胃癌细胞的增殖
Full-Text
