干扰素对JAK2 V617F阳性骨髓增殖性肿瘤PD-1/PD-L1及Treg表达的影响
DOI: 10.3971/j.issn.1000-8578.2019.18.0937
Keywords: 程序性死亡受体-1,程序性死亡配体-1,调节性T细胞,骨髓增殖性肿瘤,干扰素,Effects of Adoptive Immunotherapy on T Cell Subsets of Peripheral Blood in Non-small Cell Lung Cancer Patients,Therapeutic Effect of Interferon on Chronic Myeloproliferative Neoplasm Patients,Review on CD4+T Cells in Malignant Pleural Effusion,Clinical of CD4+CD25+Foxp3+ Regulatory T Cells Expression in Peripheral Blood and#br# Cancer Tissues of Oral Squamous Cell Carcinoma Patients,Related Factors of Thrombosis in Patients with Myeloproliferative Neoplasms,Distribution of CD4+CD25+Regulatory T Cells, CD4+T and CD8+T Cells in Colorectal Carcinoma,Association of Single Nucleotide Polymorphism of Interferon-gamma Gene +874 Site and Breast Cancer,Study of IFN-β Gene Inducing Nude Mice of Human SHG44 Glioma Up-regulating of Fas and Cell Apoptosis,Synergistic Reversal of Multidrug Resistance of Leukemia Cells by α-Interferon and Cyclosporine A,Expression of Serum IL-12,IFN-γ,VEGF Level in Acute Leukemia and Its Significance,Recombinant tumor necrosis factor, interleukin-2, and interferon-γ effects on nasopharyngeal carcinoma cell in vitro and in vivo,Effect of Interferon Alpha on Endometrial Adenocarcinoma Cell Line,Effect of IFNα-2b and BCG on the Release of IL-1 by Monocytes and Macrophages in RAT with Hepatoma,Effects of interferon-αliposome on lung metastasis of Lewis lung carcinoma and proliferation of splenocytes in mice
Abstract:
摘要 目的 探讨干扰素-alpha-2b（IFN-α2b）对JAK2 V617F阳性骨髓增殖性肿瘤（MPN）患者中程序性死亡受体-1（PD-1）、程序性死亡配体-1 （PD-L1）及CD4+ CD25+ Foxp3+调节性T细胞（Treg）表达的影响及临床意义。方法 收集JAK2 V617F阳性MPN患者61例，包括初治组41例、IFN-α2b治疗组20例，健康对照组20例。应用荧光定量PCR检测JAK2 V617F/JAK2突变率，流式细胞术检测PD-1、PD-L1、Treg的表达情况。选取15例患者骨髓及外周血标本进行体外细胞培养，应用1×106 U/L IFN-α2b作用48 h后检测PD-1、PD-L1及Treg表达情况。结果 初治组的JAK2 V617F、PD-1、PD-L1及Treg表达明显高于IFN-α2b治疗组及对照组（均P<0.05）。JAK2 V617F突变量≥50%患者骨髓髓系细胞PD-1、PD-L1及外周血Treg细胞均明显高于突变量<50%患者（均P<0.05）。相关性分析结果显示JAK2 V617F突变量与骨髓髓系细胞PD-1、PD-L1和淋巴细胞PD-1呈正相关，与Treg表达无相关性。1×106 U/L IFN-α2b作用48 h后能够体外抑制MPN原代细胞PD-1、PD-L1及Treg的表达（P<0.05）。结论 PD-1、PD-L1及Treg共同参与了MPN的发病过程，干扰素能够不同程度抑制MPNJAK2 V617F、PD-1、PD-L1及Treg表达，进而抑制MPN的进展
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