紫杉醇和顺铂对EGFR野生型与突变型肺腺癌细胞的杀伤作用比较及其分子机制
DOI: 10.3971/j.issn.1000-8578.2016.03.006
Keywords: 肺腺癌,EGFR,紫杉醇,顺铂,化疗相关基因,Clinical Analysis of Radiotherapy Combined with Cisplatin-based Chemotherapy for Locally Advanced Esophageal Squamous Cell Carcinoma,Effect of Cisplatin Combined with Buzhong Yiqi Decoction on survivin Expression in Transplantation Tumor of Nude Mice with A549/DDP Cells,Expressions of MACC1 and c-Met Proteins in Lung Adenocarcinoma and Their Correlation with Postoperative Recurrence,Experiment of Different Administration Sequences of Apatinib and Paclitaxel on Lung Cancer,Predictive Factors for Customizing Chemotherapy on Advanced Lung Adenocarcinoma,GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP,Knockdown of PKD1 Decreased Sensitivity of Human Salivary Gland Adenoid Cystic Carcinoma Cell Line ACC2 to Paclitaxel,Efficacy and Safety of Weekly Liposome-paclitaxel or Oxaliplatin Combined with Tegafur on Elderly Advanced Gastric Cancer,Mechanism of Jinlong Capsule Reversing Paclitaxel Resistance of A549 Cells Investigated by System Biology Technology,Role of IL-6 in Progress of Estrogen Promoting Lung Adenocarcinoma in Mice and Its Mechanism,Effect of siRNA-mediated notch1 Gene Silencing on Apoptosis and Radiosensitivity of Glioma Cell Line U87-EGFRvⅢ,Clinical Significance of Immunohistochemistry in Detecting Epidermal Growth Factor Receptor Mutation in Lung Cancer,Establishment of DDP Resistant Variant of Triple Negative Breast Cancer Cell Line MDA-MB-231/DDP and Its Appraisal,Valuation of SUVmax, Ki-67, p53 and EGFR in Predicting Effect of Neoadjuvant Chemotherapy on Triple-negative Breast Cancer Patients,Relationship Between FoxQ1 and Epidermal Growth Factor Receptor Gene Expression in Colorectal Cancer
Abstract:
摘要 目的 探讨化疗相关基因表达水平与EGFR突变状态的关系及其对化疗疗效的影响。方法 采用MTT法分别检测紫杉醇、顺铂对A549与HCC827细胞的24 h半数抑制浓度（IC50）；比较紫杉醇、顺铂单独及联合作用于A549与HCC827细胞杀伤作用的差异；荧光定量PCR法检测紫杉醇、顺铂作用前后A549与HCC827细胞化疗相关基因（BRCA1、ERCC1、TUBB3）的表达水平。结果 紫杉醇对A549与HCC827细胞24 h的IC50分别为100和70 μg/ml，顺铂对两种细胞的IC50分别为40和50 μg/ml。单独作用时，紫杉醇对HCC827细胞的杀伤作用明显高于A549细胞（P<0.05）；顺铂对A549细胞的杀伤作用明显高于HCC827细胞（P<0.05）；紫杉醇和顺铂联合作用对A549和HCC827细胞的杀伤作用差异无统计学意义（P>0.05），且均表现为单纯相加作用（Q=1.03, 1.06）。HCC827细胞中BRCA1基因表达明显高于A549细胞（P<0.05）；顺铂作用于A549细胞后，其ERCC1基因表达明显升高（P<0.05）。结论 紫杉醇、顺铂单独作用对EGFR野生型与突变型肺腺癌细胞杀伤作用有明显差异，而两者联合无明显差异，可能与BRCA1基因的表达有关；EGFR野生型比突变型肺腺癌细胞更容易对顺铂出现耐药
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