UHRF1在胃癌侵袭和转移中的作用及其机制
DOI: 10.3971/j.issn.1000-8578.2016.04.004
Keywords: A Case Report,Correlation Between Skull Base Invasion and Bone Metastases in Locally Advanced Nasopharyngeal Carcinoma Patients,Upper Gastrointestinal Carcinomas Incidence from 2003 to 2012 in Cixian, Hebei Province,1:2 Matched Case-control Investigation on Risk Factors of Gastric Cancer in Xianyou County,Prognostic Value of Circulating Tumor Cells for Metastatic Prostate Cancer: A Metaanalysis,UGCG is Involved in Oxaliplatin Resistance Mechanism of Human Colon Cancer Through Regulating MDR1/P-gp Expression,Relationship of Expression Difference of miR-658 and miR-492 with Pelvic Lymph Node Metastasis from Squamous Carcinoma of Cervix,Correlation of ChREBP mRNA Expression and Its CpG Island Methylation in Human Hepatocellular Carcinoma,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway
Abstract:
摘要 目的 探讨甲基化调节因子UHRF1在胃癌转移过程中的作用及其调控机制。方法 RT-PCR和Western blot检测胃癌高低转移细胞系中UHRF1的表达，应用UHRF1的siRNA和质粒改变UHRF1的表达，Transwell及体内转移实验检测胃癌转移能力的变化。甲基化特异性PCR和Western blot检测UHRF1表达改变后p16INK4A和RB1的甲基化状态及蛋白表达。结果 UHRF1在高转移潜能的胃癌细胞系GC9811-P中的表达显著高于低转移潜能的细胞系GC9811。下调UHRF1的表达抑制胃癌细胞的体外和体内转移能力，同时减弱p16INK4A甲基化，增加p16INK4A蛋白表达。上调UHRF1的表达可见相反结果。结论 UHRF1可能通过调控p16INK4A和RB1的甲基化促进胃癌细胞的侵袭和转移，其有望成为胃癌转移预警和治疗的新靶点
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