miR-1269a调控HOXD10基因抑制胆管癌细胞侵袭的作用及其机制
DOI: 10.3971/j.issn.1000-8578.2018.18.0175
Keywords: 胆管细胞癌,miR-1269a,HOXD10,侵袭,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein,Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells,Role of Gadd45 Protein Family in Tumorigenesis and Development,miR-145 Attenuates Migration and Invasion of Non-small Cell Lung Cancer A549 Cells by Targeting AP4,shRNA Interferes with Cystatin C to Inhibit Invasion and Migration of Non-small Cell Lung Cancer A549 Cells,miR-198 Targets MAPK1 to Regulate Proliferation, Apoptosis and Invasion of Cervical Cancer HeLa Cells
Abstract:
摘要 目的 探讨miR-1269a对HOXD10基因的调控作用及对胆管癌细胞侵袭能力的影响。方法 预测并筛选出调控HOXD10基因的miR-1269a作为研究对象；转染miR-1269a模拟物（mimic）及抑制剂（inhibitor）至胆管癌细胞系后检测 HOXD10mRNA及蛋白的表达变化，并观察miR-1269a对胆管癌细胞侵袭能力的影响。双荧光素酶报告基因实验验证miR-1269a与HOXD10之间的靶向作用关系。结果 miR-1269a在胆管癌组织中表达显著高于癌旁组织（P=0.0023）；miR-1269a模拟物可显著降低胆管癌细胞中HOXD10 mRNA（Mz-CHA-1: P=0.0025; RBE: P=0.0038）及蛋白表达水平，且细胞的侵袭能力较对照组显著增强（Mz-CHA-1: P=0.004; RBE: P=0.004）。miR-1269a抑制剂转染则出现相反的结果（QBC939: P=0.16; HCCC9810: P=0.13）。miR-1269a明显抑制野生型HOXD10-3’UTR 的荧光素酶活性，而对突变型质粒转染细胞的荧光素酶活性无影响。结论 miR-1269a靶向负性调控HOXD10基因，在胆管癌细胞侵袭过程中发挥重要作用
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