沉默YAP通过Wnt/β-catenin信号通路影响胃癌BGC823细胞的凋亡
DOI: 10.3971/j.issn.1000-8578.2018.18.0495
Keywords: 胃癌,YAP,凋亡,Wnt/β-catenin,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma
Abstract:
摘要 目的 本研究探讨Yes相关蛋白（YAP）对胃癌BGC823细胞凋亡的影响。方法 BGC823细胞中转染YAP小干扰RNA（YAP siRNA1、YAP siRNA2），用qRT-PCR和蛋白质印迹法分别测定细胞中YAP表达，筛选干扰效果较好的YAP siRNA1做后续实验。MTT测定细胞增殖，平板克隆实验测定克隆形成能力，流式细胞术测定凋亡情况，分光光度法检测Caspase-3、Caspase-9活性，蛋白质印迹法检测β-catenin、c-myc、CyclinD1蛋白水平。用Wnt/β-catenin的激活剂处理下调YAP后的BGC823细胞，按照上述方法测定细胞增殖凋亡和克隆形成能力。结果 YAP siRNA1、YAP siRNA2下调BGC823细胞中YAP的转录和表达，YAP siRNA1对YAP表达的干扰效率较好。敲低YAP后的细胞增殖能力、克隆形成能力降低，细胞凋亡率由（6.32±0.58）%升高至（32.71±2.64）%，Caspase-3、Caspase-9活性升高，β-catenin、c-myc、cyclinD1蛋白表达减少，与正常培养的BGC823细胞相比，差异有统计学意义（P<0.05）。Wnt/β-catenin的激活剂处理可以部分逆转敲低YAP对BGC823细胞的凋亡、增殖、克隆形成能力和细胞中Caspase-3、Caspase-9活性的影响。结论 YAP敲低诱导胃癌细胞凋亡，且作用机制与抑制Wnt/β-catenin通路激活有关
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