Silver Nanoparticles: Cytotoxic and Apoptotic Activity On HT-29 and A549 Cell Lines - Silver Nanoparticles: Cytotoxic and Apoptotic Activity On HT-29 and A549 Cell Lines - Open Access Pub

Silver nanoparticles (Ag-NPs) are versatile materials with a broad range of applications in various fields such as cancer therapy, drug delivery. In this work, cytotoxic and apoptotic activities of silver nanoparticles was evaluation against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed by MTT assay, while their apoptotic activity was tested through TUNEL method. The results of MTT of A549 have illustrate that fifty percent of cells destruction in concentrations more than 250 μg/ml of Ag-NPs. Apoptotic results of nanoparticles have shown more than fifty percent of apoptosis on A549 cell line. HT-29 display full apoptosis at concentrations more than 500 μg/ml. It seems that synthesized Ag-NPs by using P. farcta extract can be candidate as anti-cancer agent in treatment many cancers through creating or discovering new drug forms DOI 10.14302/issn.2377-2549.jndc-18-2116 In recent years, nanoparticles have developed as diagnostic and therapeutic agents for the treatment of many diseases such as diabetes, asthma, allergies and cancer 1. Ag-NPs have special physicochemical properties, which apply such as solar cells, catalyst in chemistry reaction, electric batteries etc. 1. Also, they have contain antimicrobial 2, antiviral 3, antifungal 4, 5, anti-inflammatory 6, and antioxidant effects 7. Studies have showed the usage of nanoparticles in drug delivery and molecular imaging 8, while researches have reported their capability in eliminating cancer cells 9, 10. Some cancer cells have been reported to show resistance toward many drugs, specially platin derivatives such as cis-platin, as well as showing toxic effects. Thus, it is vital to discover new drugs for this particular disease in which Ag-NPs seem to fit as a suitable suggestion 11. Recent studies on Ag-NPs have shown significant anti-cancer activity on breast (MCF-7) 9, mouse fibroblasts (L929) 8, colon (HCT-119) 12, lung (A549) 13, pharynx (Hep-2) 14, rat skeletal muscle (L6) 15, and prostate (MDA-MB-231) 16 cancer cell lines. The aim of this project is to investigate the anticancer activities of synthesized Ag-NPs by using Prosopis farcta extract against lung (A549) and colon (HT-29) cancer cell lines. These nanoparticles, which contain spherical shapes with size about 10.8 nm 17, have been already synthesized by our team members. The cytotoxic and apoptotic activities of the mentioned nanoparticles were investigated through the use of MTT assay and TUNEL test, respectively. Synthesis of Ag-NPs Ag-NPs were synthesized according to the protocol of Miri