The Antioxidant and Hepatoprotective Activities of Two Tea Polysaccharides - The Antioxidant and Hepatoprotective Activities of Two Tea Polysaccharides - Open Access Pub

In the present study, we investigated the chemical compositions, in vitro antioxidant and in vivo hepatoprotective activities of two tea polysaccharides (TPS), which were extracted from two different tea cultivars, Yingshuang (Camellia Senesis, T01) and Yunnan Dayezhong (Camellia Senesis, T09). Compared with T09-TPS, T01-TPS had lower contents of neutral sugar, protein, uronic acid and polyphenol. However, T01-TPS showed stronger scavenging abilities for transient free radicals of hydroxyl radical and superoxide anion radicals and lipid peroxidation inhibition effect, but weaker scavenging ability for stable free radical of DPPH. For hepatoprotective activity in vivo, the results demonstrated that both T01-TPS and T09-TPS could significantly prevent the increase of serum alanine aminotransferase and, aspartate aminotransferase levels, decrease the liver index, reduce the formation of malonydialdehyde and enhance the activities of superoxide dismutase, glutathione peroxidase and peroxidase in carbon tetrachloride-induced liver injury mice. These results suggest that T01-TPS and T09-TPS have potent antioxidant and hepatoprotective activities. DOI 10.14302/issn.2471-2140.jaa-17-1541 Tea is the most widely consumed beverage in the world and also an important agricultural product in some developing countries. Many researches have revealed that tea possesses a wide range of beneficial effects including reduction of cholesterol, depression of hypertension, anti-oxidation and anti-cancer (Ganguly, 2017)1. These beneficial effects are partly attributed to the chemical ingredients in tea leaves, including polyphenols, polysaccharides, alkaloids and amino acids, etc. (Nie and Xie, 2011); Wang et al., 2012)13,15 Polysaccharides have attracted much attention due to their biological activities in the past two decades. Tea polysaccharides(TPS), one of the main components of tea extracts, have demonstrated beneficial properties in anti-oxidation, reducing blood sugar activities(Zhou, Xie & Fu, 2001a; Xie & Nie, 2006; Wang, Wang, & Li, 2001). 35 TPS have active effects on scavenging reactive oxygen species (ROS) including hydroxyl radical, superoxide anion and 1, 1-Diphenyl-2-picryldydrazyl (DPPH) radical in vitro. In addition, a recent report showed that tea polysaccharides extracted from leaf, seed and flower exhibited different antioxidant abilities in vitro(Wang et al., 2012).15 The cultivar is one of the important factor affecting the components and contents of TPS. Our laboratory have determined the free radical scavenging abilities of TPS extracted from 209 tea