Profile of Similarity of Electron Withdrawing Structure Towards Analgesic-anti-inflammatory Activity of the Novel Isatin Analogue: Design and Implementation of Phase I Drug Discovery - Profile of Similarity of Electron Withdrawing Structure Towards Analgesic-anti-inflammatory Activity of the Novel Isatin Analogue: Design and Implementation of Phase I Drug Discovery - Open Access Pub

Isatin (1H-indole-2,3-dione ) and derivatives demonstrate a diverse array of biological activities. Isatin and 5-halo derivatives has reacted to form the schiff’s bases , mannich bases and friedal craft alkylation’s to form C-C, C-N, C=N bonds. From the spectral studies, isatin has undergoes reaction at C-3 and N-1 position and synthesized lead in present schme and seen the similarity of structure and analgesic-anti-inflammatory activity. DOI10.14302/issn.2578-8590.ipj-18-2113 Isatin is a versatile compound isolated in 1988 and reported to possess a wide range of biological activity in mammals. Isatin also is a synthetically versatile substrate that can be used to prepare a large variety of heterocyclic compounds, such as indoles and quinolines, and as a raw material for drug synthesis. Schiff bases and mannich bases of isatin has known to possess a wide range of pharmacological properties including anticonvulsant, antibacterial, antiprotozoal, antifungal, antiviral, anti-HIV, tuberculostatic, analgesic, anticancer activities. In the view of this fact, we have planned the synthesis of some novel isatin analogue and screening them for analgesic-anti-inflammatory activity. Isatin, an extract from Strobilanthes cusia. The roots and the leaves of the plant, Strobilanthes cusia of the Acanthaceae family that is widely distributed in northern and central China, have been used in traditional Chinese medicine to treat a variety of ailments caused by microorganisms and virus. Material and Methods All reagents have obtained from Sigma Aldrich Bangalore, and Loba Chemie, Mumbai. All the solvents used in these studies has dried and distilled before use. Melting points have determined by open capillary tube method and are uncorrected. The purity and homogeneity of the synthesized compound has ascertained by T.L.C. on glass plate using silica gel G as adsorbent and solvent system benzene: ethanol (9:1). The spot has visualized by iodine vapor. The 1H-NMR spectra has recorded on CDCl3 (BRUCKRS). All spectra have obtained from Indian Institute of Technology Pawai Mumbai. Methodology Scheme 1] Synthesis of 5-substituted-indole-2, 3 dione (Isatin) SubstitutedIsatin (Scheme I) 2] Method of preparation of 5-substituted N-benzyl isatin derivatives Where,R1=H,Cl,F,NO2 (Scheme II) 3] Methods of preparation of Schiff’s bases of 5-substituted N-benzyl isatin derivatives: (Scheme III) Lab Scale R & D Methods of Preparation of 5-substituted N-benzyl isatin derivatives: 6 Equimolecular quantity of isatin and benzyl chloride has taken in RBF. To this mixture 20 ml of dimethyl