Total Proteins, β- and γ-globulins As Efficacy Therapy Response Indicators in Dogs Infected With Leishmania Infantum – A Review - Total Proteins, β- and γ-globulins As Efficacy Therapy Response Indicators in Dogs Infected With Leishmania Infantum – A Review - Open Access Pub

Leishmaniasis treatment monitoring is an important problem, since patient’s frequently present clinical signs improvements with positive indirect immuno？uorescence (IFI) titers of anti-Leishmania antibodies, thus making difficult the clinician understand the therapy efficacy. The study aimed 1) to identify over a short period of 30 days, which of the main changes on the serum proteinogram fractions in patients treated with meglumine antimoniate and allopurinol, can be pointed as indicator to classify patients as slower or faster responsive to treatment. A sample of 56 dogs (n=56) with leishmaniasis diagnosis was followed-up for clinical condition, proteinograms and titers of anti-Leishmania antibodies during the treatment period considering three different time points: M0 (diagnosis moment), M1 (15 days after therapy start), and M2 (30 days after therapy start). Two groups of patients were considered according to their clinical condition evolution rate: faster recovery group (FRG) and slower recovery group (SRG). Statistical significant results were considered for p-value <0.05. Statistically significant differences in proteinogram variations between FRG and SRG were registered for TPs (p= 0.03), and for the fractions β (p=0.04), γ (p=0.04), amongst M0 and M2.The PT, β and γ-globulin fractions of proteinogram, in association with patient clinical assessment evolution should be considered as an indicator and a simple way to appoint the efficacy response of the patients to the therapy. DOI10.14302/issn.2575-1212.jvhc-17-1764 Leishmaniasis treatment monitoring is an important problem, since patient’s frequently present clinical signs improvements with positive indirect immuno？uorescence (IFI) titers of anti-Leishmania antibodies, thus making difficult the clinician understand the therapy efficacy. Proteinogram has potential for monitoring the patient response to leishmaniasis therapy (Noli 1999, Martínez-Subiela & Cerón 2005, Cerón, Caldin, Martinez-Subiela 2011, Paim et al. 2013). Electrophoresis is designed to separate serum protein into different fractions, and it is the result of serum exposing to an electrical current allowing obtain the different serum proteins which migrate in bands, separating and quantifying the different fractions in a sample, according to their physical properties, such as size, charge and molecular weight (Eckersal 2008), the proteinogram in domestic animals considers the following fractions: albumin, alpha (α) globulin (α1, α2), beta (β) globulin (β1, β2), and gamma (γ) globulin (Thomas 2000, Meyer, Harvey 2004, Eckersal