Bisphenol A Down-regulates the Mrna expression of steroidogenic genes and induces histopathological changes in testes of rats Expression of Steroidogenic Genes and Induces Histopathological Changes in Testes of Rats - Bisphenol A Down-regulates the Mrna expression of steroidogenic genes and induces histopathological changes in testes of rats Expression of Steroidogenic Genes and Induces Histopathological Changes in Testes of Rats - Open Access Pub

Bisphenol A (BPA) is an endocrine disruptor with a weak estrogenic effect used in industry as a component of food cans. We aimed to study the toxic effects of BPA on mRNA expression of steroidogenic genes and testicular structure in mature male rats. Animals were divided into 3 groups: vehicle control rats as first group, while second group received 10 μg/kg BW and third group received BPA 15 μg/kg BW orally every alternate day for a period of 105 successive days. Serum testosterone level, mRNA expression of genes related to steroid synthesis, histopathological examination, spermatogenesis index and number of Leydig cells were evaluated in this study. Lower serum hormone levels were observed in both BPA-treated groups as compared to the control group. The gene expression patterns of steroidogenic acute regulatory protein (StAR), cytochrome P450 17a(CYP17a) and 3β-Hydroxysteroid dehydrogenase (3β-HSD) were significantly down-regulated in BPA-treated rats compared to control group. Meanwhile, the expression of aromatase (CYP19) and lutinizing hormone receptor (LHR) was significantly up-regulated. Histopathological lesions were observed in the testes and epididymis of BPA-treated rats. Spermatogenesis index and the number of Leydig cells were significantly decreased in BPA-treated groups compared with the control group. This study highlights negative effect of BPA on steroidogenic genes and testicular structure in male rats. DOI10.14302/issn.2575-1212.jvhc-18-2012 Bisphenol A (BPA) is a ubiquitous environmental chemical that is integrated in the manufacturing process of many industrial products such as plasticizers, as well as in the production of materials used for food and potable water, such as epoxy lining of food and beverages cans 1, 2, 3, 4. It leaches from those products causing health hazards for humans and animals 2. Its presence in biological and nonbiological samples was previously verified 5. BPA is an endocrine disruptor; it binds to estrogenic receptors mimics the action of estrogen hormone 6, also, it can bind to androgen receptors, blocking endogenous androgen action 7 resulting in impairment of male reproductive function 8, 9. BPA at a dose of 10 mg/kg bw/day orally for 14 days reduced activity of testicular mitochondrial enzymes in micewith subsequent stimulation of oxidative stress through inhibiting the activities of antioxidant enzymes 10. Alternatively, extremely low concentrations of BPA inhibited activity of steroidogenic enzymes in human and rat testicular microsomes 11. Reproductive toxicity of BPA was previously studied using