Antioxidant Protection of Donor Packed Red Blood Cells Using Mexidol - Antioxidant Protection of Donor Packed Red Blood Cells Using Mexidol - Open Access Pub

The current research shows the possibility of long-term conservation of the erythrocytes an-tioxidant activity in the process of donor packed red blood cells while introducing mexidol to the hemoconservant glugicir composition. DOI10.14302/issn.2471-2140.jaa-18-2283 Storage and supply of blood components for possible emergency disaster situation is one of the main tasks of emergency disaster medicine. While donor blood and its components storage, the viability, morphological and functional values of erythrocytes are gradually decreasing. Alterations in structural and functional organization of cells membranes during donor blood components storage and failure in functioning of membrane connected enzymes and excessive synthesis of free radicals inevitably leads to accelerated blood cells “ageing” 1, 2. Hence, correction of pathophysiological changes in blood transfusion environment at the stage of blood storage is our priority task 3. We consider creation of new hemopreservatives and further development of the existing ones as the most important trends in transfusiology which allows raising functional value of erythrocytes, medical efficiency of blood transfusions, and lasting blood components storage time 4, 5. The overall aim of this research is to investigate the capacity of long-termpreservation of the erythrocyte native state in donor packed red blood cells (RBC) by inactivating the processes of lipid peroxygenation (LP) and correction of antioxidant activity (AAC) in erythrocytes with standard RBC storage (at t + 4 0С), by adding 2-ethyl-6-methyl-3-hydroxypyridine succinate antioxidant to composition of the blood conversant “Glugicir”. We prepared several samples of packed RBC on glugicir preservative which contained 0.25 mg/ml, 0.5 mg/ml and 1.0 mg/ml 2-ethyl-6-methyl-3-hydroxypyridine succinate antioxidant, respectively. As a control sample we used packed RBC prepared on glugicir with no supplements. We examined the biochemical values of erythrocites in the packed RBC samples immediately after their preparation and in during its storaging at the t = + 4 0С for 6 hours, 3 days, 7 days, 14 days, 21 days and 30 days. The AAC intensity has been estimated by concentration of malondialdehyde (MDA), which was defined by the reaction with 2-thiobarbituric acid, according to the L.I. Andreeva’s method 6. The intensity of the Free Radical Oxidation (FRO) and General Antioxidant Activity (GAA) were assessed by the chemiluminometer Emilite-1105 (Latvia). The level of catalase was defined according to the M.A. Koroluk’s and Co method) 7. The method based on