We developed a Bessel-beam photoacoustic microscopical simulation platform by using the k-Wave: MATLAB toolbox. The simulation platform uses the ring slit method to generate Bessel beam. By controlling the inner and outer radius of the ring slit, the depth-of-field (DoF) of Bessel beam can be controlled. And the large volumetric image is obtained by point scanning. The simulation experiments on blood vessels were carried out to demonstrate the feasibility of the simulation platform. This simulation work can be used as an auxiliary tool for the research of Bessel-beam photoacoustic microscopy.
References
[1]
Wang, L.V. and Hu, S. (2012) Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs. Science, 335, 1458-1462.
https://doi.org/10.1126/science.1216210
[2]
Wang, L.V., Wang, X., Ku, G. and Stoica, G. (2004) High-Resolution Functional Photoacoustic Tomography. IEEE International Symposium on Biomedical Imaging from Nano to Macro, 1, 1479-1481.
[3]
Yao, J. and Wang, L.V. (2011) Photoacoustic Tomography: Fundamentals, Advances and Prospects. Contrast Media Mol. Imaging, 6, 332–345.
https://doi.org/10.1002/cmmi.443
[4]
Wang, L.V. (2008) Prospects of Photoacoustic Tomography. Medical Physics, 35, 5758-5767. https://doi.org/10.1118/1.3013698
[5]
Hu, S. and Wang, L.V. (2010) Photoacoustic Imaging and Characterization of the Microvasculature. Journal of Biomedical Optics, 15, 011101.
https://doi.org/10.1117/1.3281673
[6]
Wang, X., Pang, Y., Ku, G., Xie, X., Stoica, G. and Wang, L.V. (2003) Noninvasive Laser-Induced Photoacoustic Tomography for Structural and Functional in Vivo Imaging of the Brain. Nature Biotechnology, 21, 803-806.
https://doi.org/10.1038/nbt839
[7]
Zhang, H.F., Maslov, K., Stoica, G. and Wang, L.V. (2006) Functional Photoacoustic Microscopy for High-Resolution and Noninvasive in Vivo Imaging. Nature Biotechnology, 24, 848-851. https://doi.org/10.1038/nbt1220
[8]
Liu, Y., Zhang, C. and Wang, L.V. (2012) Effects of Light Scattering on Optical-Resolution Photoacoustic Microscopy. Journal of Biomedical Optics, 17, Article ID: 126014. https://doi.org/10.1117/1.JBO.17.12.126014
[9]
Maslov, K., Zhang, H.F., Hu, S. and Wang, L.V. (2008) Optical-Resolution Photoacoustic Microscopy for in Vivo Imaging of Single Capillaries. Optics Letters, 33, 929-931. https://doi.org/10.1364/OL.33.000929
[10]
Yao, J., Wang, L., Yang, J.M., Maslov, K.I., Wong, T.T.W., Li, L., Huang, C.H., Zou, J. and Wang, L.V. (2015) High-Speed Label-Free Functional Photoacoustic Microscopy of Mouse Brain in Action. Nature Methods, 12, 407-410.
https://doi.org/10.1038/nmeth.3336
[11]
Yeh, C., Soetikno, B., Hu, S., Maslov, K.I. and Wang, L.V. (2014) Microvascular Quantification Based on Contour-Scanning Photoacoustic Microscopy. Journal of Biomedical Optics, 19, Article ID: 096011.
https://doi.org/10.1117/1.JBO.19.9.096011
Hajireza, P., Forbrich, A. and Zemp, R.J. (2013) Multifocus Optical-Resolution Photoacoustic Microscopy Using Stimulated Raman Scattering and Chromatic Aberration. Optics Letters, 38, 2711-2713. https://doi.org/10.1364/OL.38.002711
[14]
Li, B., Qin, H., Yang, S. and Xing, D. (2014) In Vivo Fast Variable Focus Photoacoustic Microscopy Using an Electrically Tunable Lens. Opt. Express., 22, Article ID: 020130. https://doi.org/10.1364/OE.22.020130
[15]
Shi, J., Wang, L., Noordam, C. and Wang, L.V. (2015) Bessel-Beam Grueneisen Relaxation Photoacoustic Microscopy with Extended Depth of Field. Journal of Biomedical Optics, 20, Article ID: 116002. https://doi.org/10.1117/1.JBO.20.11.116002
[16]
Treeby, B.E. and Cox, B.T. (2010) k-Wave: MATLAB Toolbox for the Simulation and Reconstruction of Photoacoustic Wave Fields. Journal of Biomedical Optics, 15, 021314. https://doi.org/10.1117/1.3360308