Synthesis of silver nanoparticles using seeds of Nigella sativa as a capping agent was evaluated in this study. Different concentrations of the aqueous extract of N. sativa with silver nitrate solution were exposed to sunlight; as a force for acceleration of the formulation. Then the silver nanoparticles were characterized by UV-Vis, scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. Antibacterial activity of the nanoparticles was investigated against Staphylococcus aureus and Escherichia coli by the disc diffusion method. The characterization of nanoparticles was detected by the change in color to yellow-brown which indicated the formulation of silver nanoparticles. Irregular shapes within range of nanoscale were detected using SEM and XRD techniques. The finding suggests that silver nanoparticles may be effectively used as antibacterial agent.
References
[1]
Rogers, B., Adams, J. and Pennathur, S. (2014) Nanotechnology: Understanding Small Systems. CRC Press, Boca Raton.
[2]
Woodson, T.S. (2016) Public Private Partnerships and Emerging Technologies: A Look at Nanomedicine for Diseases of Poverty. Research Policy, 45, 1410-1418. https://doi.org/10.1016/j.respol.2016.04.005
[3]
Bawa, R. (2016) What’s in a Name? Defining “Nano” in the Context of Drug Delivery. In: Handbook of Clinical Nanomedicine: Nanoparticles, Imaging, Therapy, and Clinical Applications, Pan Stanford Publishing, Singapore, 127-168. https://doi.org/10.1201/b19915
[4]
Harstad, S., Hunagund, S., Boekelheide, Z., Hussein, Z.A., El-Gendy, A.A. and Hadimani, R.L. (2018) Gd-Based Magnetic Nanoparticles for Biomedical Applications. In: Magnetic Nanostructured Materials, Elsevier, Amsterdam, 137-155. https://doi.org/10.1016/B978-0-12-813904-2.00005-X
[5]
Veisi, H., Pirhayati, M., Kakanejadifard, A., Mohammadi, P., Abdi, M.R., Gholami, J. and Hemmati, S. (2018) In Situ Green Synthesis of Pd Nanoparticles on Tannic Acid-Modified Magnetite Nanoparticles as a Green Reductant and Stabilizer Agent: Its Application as a Recyclable Nanocatalyst (Fe3O4@ TA/Pd) for Reduction of 4-Nitrophenol and Suzuki Reactions. Chemistry Select, 3, 1820-1826. https://doi.org/10.1002/slct.201702869
[6]
Zhu, X., Pathakoti, K. and Hwang, H.M. (2019) Green Synthesis of Titanium Dioxide and Zinc Oxide Nanoparticles and Their Usage for Antimicrobial Applications and Environmental Remediation. In: Green Synthesis, Characterization and Applications of Nanoparticles, Elsevier, Amsterdam, 223-263. https://doi.org/10.1016/B978-0-08-102579-6.00010-1
[7]
Singh, J., Dutta, T., Kim, K.H., Rawat, M., Samddar, P. and Kumar, P. (2018) “Green” Synthesis of Metals and Their Oxide Nanoparticles: Applications for Environmental Remediation. Journal of Nanobiotechnology, 16, 84. https://doi.org/10.1186/s12951-018-0408-4
[8]
Siddiqi, K.S., Husen, A. and Rao, R.A. (2018) A Review on Biosynthesis of Silver Nanoparticles and Their Biocidal Properties. Journal of Nanobiotechnology, 16, 14. https://doi.org/10.1186/s12951-018-0334-5
[9]
McGillicuddy, E., Murray, I., Kavanagh, S., Morrison, L., Fogarty, A., Cormican, M., Morris, D., et al. (2017) Silver Nanoparticles in the Environment: Sources, Detection and Ecotoxicology. Science of the Total Environment, 575, 231-246. https://doi.org/10.1016/j.scitotenv.2016.10.041
[10]
Patel, M.S. (2019) Inactivation of Antibiotic Resistant Bacteria and Genes by Conventional and Advanced Disinfection Methods. Doctoral Dissertation, Rice University, Houston.
[11]
Gambino, D. and Otero, L. (2019) Metal Compounds in the Development of Antiparasitic Agents: Rational Design from Basic Chemistry to the Clinic. In: Essential Metals in Medicine: Therapeutic Use and Toxicity of Metal Ions in the Clinic, De Gruyter, Berlin, Boston, 19, 331. https://doi.org/10.1515/9783110527872-013
[12]
Mittal, R., Pan, D.R., Parrish, J.M., Huang, E.H., Yang, Y., Patel, A.P., Harjai, K., et al. (2018) Local Drug Delivery in the Urinary Tract: Current Challenges and Opportunities. Journal of Drug Targeting, 26, 658-669. https://doi.org/10.1080/1061186X.2017.1419356
[13]
Ranjan, P., Das, M.P., Kumar, M.S., Anbarasi, P., Sindhu, S., Sagadevan, E. and Arumugam, P. (2013) Green Synthesis and Characterization of Silver Nanoparticles from Nigella sativa and Its Application against UTI Causing Bacteria. Journal of Academia and Industrial Research, 2, 45-49.
[14]
Safain, K.S. (2017) Screening of Silver Resistance Gene in Clinical Isolates and Determination of Minimum Inhibitory Concentration (MIC) for Silver Nitrate. Doctoral Dissertation, BRAC University, Dhaka.
[15]
Sharma, V., Chotia, C., Ganesan, V. and Okram, G.S. (2017) Influence of Particle Size and Dielectric Environment on the Dispersion Behaviour and Surface Plasmon in Nickel Nanoparticles. Physical Chemistry Chemical Physics, 19, 14096-14106. https://doi.org/10.1039/C7CP01769C
[16]
Amooaghaie, R., Saeri, M.R. and Azizi, M. (2015) Synthesis, Characterization and Biocompatibility of Silver Nanoparticles Synthesized from Nigella sativa Leaf Extract in Comparison with Chemical Silver Nanoparticles. Ecotoxicology and Environmental Safety, 120, 400-408. https://doi.org/10.1016/j.ecoenv.2015.06.025
[17]
Tafur, G., Benndorf, C., Acosta, D., Asencios, J. and Talledo, A. (2019) Optical Properties of Silver Nanoparticles Embedded in Dielectric Films Produced by dc and rf Magnetron Sputtering. Journal of Physics: Conference Series, 1173, Article ID: 012004. https://doi.org/10.1088/1742-6596/1173/1/012004
[18]
Aumeeruddy, M.Z., Zengin, G. and Mahomoodally, M.F. (2018) A Review of the Traditional and Modern Uses of Salvadora persica L. (Miswak): Toothbrush Tree of Prophet Muhammad. Journal of Ethnopharmacology, 213, 409-444. https://doi.org/10.1016/j.jep.2017.11.030
