基于数字全息显微技术的生物细胞动态定量测量
Dynamic Quantitative Measurement of Cells Based on Digital Holographic Microscopy

DOI: 10.12677/OE.2019.92012, PP. 77-82

Keywords: 数字全息显微，光程长，动态测量
Digital Holographic Microscopy, Optical Path Length, Dynamic Measurement

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Abstract:

对生物细胞的动态测量已长期停留在定性观察的基础上。细胞作为透明样本，其透射光程长具有与细胞形态、细胞质浓度线性相关的特点，因此可定量反映细胞的生命状态。数字全息显微技术能够快速获得样本的定量动态光程长信息，并且不会对样本造成损害。本文利用透射式数字全息显微系统对海拉细胞和大肠杆菌进行动态观察，获取透射光程长，分析光程长信息与生命过程的映射关系，为探索环境对生物细胞的影响提供一种新型的定量测量手段。
The dynamic measurement of biological cells has been based on the qualitative observations for a long time. As a transparent sample, a cell’s optical path length is linearly related to cell morphology and cytoplasm concentration. As a result, the living state of a cell can be reflected by the optical path length quantitatively. Optical path length can be obtained by digital holographic microscopy without damaging the sample. In this paper, we used the transmission digital holographic microscopy system to observe the HeLa cells and Escherichia coli dynamically to obtain the optical path lengths. By analyzing the relationship between optical path lengths and living state of biological cells, a new quantitative measurement method is proposed to explore the influences caused by the environment.

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