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灵菌红素的纯化制备工艺研究
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Abstract:
通过微生物发酵法生产灵菌红素，薄层层析法筛选合适的灵菌红素展开剂，然后利用硅胶柱层析高效纯化制备灵菌红素纯品，高效液相色谱法检测各纯化组分中灵菌红素的含量。结果发现，微生物发酵法生产灵菌红素粗产品达到6.23 ± 1.56 g/L，其含量为0.29% ± 0.14%；丙酮提取物达到52.3 ± 4.5 mg/L，其含量为29.6% ± 4.8%；以氯仿与乙酸乙酯为基础筛选出9:1比例的灵菌红素与杂质的分离效果较好，适合作为硅胶柱层析的洗脱剂进行灵菌红素的纯化，纯化后的产品经过乙醚洗涤结晶后，产量达到3.3 ± 0.4 mg/L，其含量为96.2 ± 1.3%。
In this work, prodigiosin from microbial was purified by silica gel column chromatography, and suitable eluents for prodigiosin were screened by TLC. The content of prodigiosin in different pu-rified steps was determined by high performance liquid chromatography. The results showed that the crude product of prodigiosin produced by microbial fermentation reached 6.23 ± 1.56 g/L with the content of 0.29% ± 0.14%; the acetone extract reached 52.3 ± 4.5 mg/L with the content of 29.6% ± 4.8%; the ratio of suitable eluent was 9:1 (chloroform and ethyl acetate). The final content of prodigiosin could reach 96.2% ± 1.3% and its yield was 3.3 ± 0.4 mg/L.
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