基于均匀设计法优化骆驼蓬抗氧化性物质提取工艺
Optimization of the Extraction Technology of Antioxidant Substances from the Peganum harmala L. by Uniform Design

DOI: 10.12677/HJCB.2019.93008, PP. 52-58

Keywords: 骆驼蓬，提取溶剂，均匀设计法，DPPH抗氧化活性
Peganum harmala L., Extraction Solvent, Uniform Design Method, Antioxidant Activity of DPPH

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Abstract:

为探寻骆驼蓬中抗氧化性物质的最佳提取方法，采用不同极性溶剂提取与均匀设计法相结合进行试验。以骆驼蓬为研究对象，DPPH清除自由基抗氧化活性抑制率为测试指标，对均匀设计实验数据进行二次多项式逐步回归分析。结果筛选甲醇作为最佳提取溶剂，其抑制率为76.64%。得到最优提取工艺条件：提取3次，提取时间20 min，料液比1:14 g/mL，通过验证此时骆驼蓬的甲醇提取物DPPH清除自由基抗氧化活性的抑制率为75.13%，为最佳提取工艺。
In order to optimize the extraction process of antioxidants in Peganum harmala L., different solvent extraction combined uniform design method were employed in this research. With the inhibition rate of DPPH scavenging free radical antioxidant activity as the test index, the experimental data of uniform design were analyzed by quadratic polynomial stepwise regression. Methanol was selected to be the optimum extraction solvent with an inhibition of 76.64%. The optimum extraction conditions are as follows: extract is 3 times; extraction time is 20 min; liquid-solid ratio is 1:14 g/mL. At this time, the inhibition rate of scavenging DPPH free radical antioxidant activity of methanol extract from Peganum harmala L. was 75.13%, which was the best extraction process.

References

[1]	宋立人. 现代中药学大辞典[M]. 北京: 人民卫生出版社, 2001.
[2]	Shang, X.F., Guo, X., Li, B., et al. (2016) Microwave-Assisted Extraction of Three Bioactive Alkaloids from Peganum harmala L. and Their Acaricidal Activity against Psoroptes cuniculi in Vitro. Journal of Ethnopharmacology, 192, 350-361.
[3]	全庆华, 姬瑞芳, 袁将, 等. 骆驼蓬多糖提取及对秀丽隐杆线虫多聚谷氨酰胺聚集毒性的影响[J]. 北京中医药大学学报, 2017, 40(11): 954-959.
[4]	张普照, 王俊儒, 姚伟琴, 等. 骆驼蓬地部总黄酮提取工艺的优化[J]. 西北农业学报, 2005, 14(5): 114-117.
[5]	罗建民, 李君, 王成虎, 等. 福林法测定骆驼蓬不同部位中多酚含量[J]. 理化检验(化学分册), 2013, 49(2): 232-233.
[6]	赵婷, 王长虹, 王峥涛. 骆驼蓬属植物中生物碱类化学成分及其药理活性研究进展[J]. 国际药学研究杂志, 2010, 37(5): 333-339+345.
[7]	李凯, 薛小青, 张洪亮. 骆驼蓬有效成分的提取工艺及抗肿瘤机制研究进展[J]. 新疆中医药, 2015, 33(2): 80-82.
[8]	Roudaina, B., Lamjed, B., Adele, P., et al. (2018) Anti HSV-2 Activity of Peganum harmala (L.) and Isolation of the Active Compound. Microbial Pathogenesis, 114, 291-298. https://doi.org/10.1016/j.micpath.2017.12.017
[9]	Baky, A., Rahman, A., Mekawi, E., Ibrahem, E.A. and Shalapy, N.M. (2016) The Anti-Diabetic and Anti-Lipidemic Effects of Peganum harmala Seeds in Diabetic Rats. Der Pharmacia Lettre, 8, 1-10. https://doi.org/10.1155/2016/7389864
[10]	刘俊玲, 乌庆超, 王朝晖, 等. 去氢骆驼蓬碱诱导急性白血病Jurkat细胞凋亡及其机制[J]. 光明中医, 2012, 27(2): 242-244.
[11]	赵静. 骆驼蓬总生物碱正交提取工艺与抗自由基活性研究[J]. 中兽医医药杂志, 2014, 33(1): 48-53.
[12]	李雪, 张鸣, 常国华, 等. 骆驼蓬粗多糖抗氧化性的研究[J]. 中国食品添加剂, 2016(1): 85-88.
[13]	Li, Y.P., He, Q., Geng, Z.F., et al. (2018) NMR-Based Metabolomic Profiling of Peganum harmala L. Reveals Dynamic Variations between Different Growth Stages. Royal Society Open Science, 5, Article ID: 171722. https://doi.org/10.1098/rsos.171722
[14]	Li, Y.P., He, Q., Du, S.S., et al. (2018) Study of Methanol Extracts from Different Parts of Peganum harmala L. Using 1H-NMR Plant Metabolomics. Journal of Analytical Methods in Chem-istry, 2018, Article ID: 6532789. https://doi.org/10.1155/2018/6532789
[15]	Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science and Technology, 28, 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
[16]	郭雪峰, 岳永德, 孟志芬, 等. 用清除羟自由基法评价竹叶提取物抗氧化能力[J]. 光谱学与光谱分析, 2010, 30(2): 508-511.
[17]	鲁静, 牛晓静, 段晓颖. 均匀设计法优选淫羊藿多糖提取工艺[J]. 中国现代中药, 2019, 21(1): 95-98.
[18]	叶峰. 运用MATLAB软件进行回归分析建模[J]. 成都航空职业技术学院学报, 2007, 23(2): 44-47.

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