月见草根多糖提取工艺优化及其抗氧化活性研究
Optimization of Extraction Process and Antioxidant Activity in Vitro for Polysaccharide from Oenothera erythrosepala Root Polysaccharide

DOI: 10.12677/BR.2019.92011, PP. 83-95

Keywords: 月见草，多糖，超声提取，微波提取，响应面，过氧化氢，RAW264.7
Oenothera erythrosepala, Polysaccharide, Ultrasonic Extraction, Microwave Extraction, The Response Surface, Hydrogen Peroxide, RAW264.7

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Abstract:

为了更好的开发月见草根中的多糖，本文运用单因素和响应面法对月见草根中多糖的最佳提取工艺进行了一系列研究。根据最佳提取工艺，对不同时期和不同土质中月见草根多糖的动态积累规律进行了的分析，并研究了月见草根中多糖对过氧化氢氧化损伤的RAW264.7细胞的修复作用。结果显示，用微波提取法提取月见草根中多糖比用超声提取法和传统工艺法有更好的提取效果。通过单因素和响应面法分析，在微波提取法中，月见草根中多糖的最佳提取条件：微波功率460 w、浸提时间25 min、料液比1:20，在此条件下的提取率为78.55%。在最佳提取方法下，对不同时期和不同土质中月见草根多糖的动态积累规律研究得知，11月份的月见草根多糖的含量最高为75.61%，而砂质中种植的月见草根的多糖含量高于壤土。抗氧化实验显示月见草多糖对过氧化氢氧化损伤的RAW264.7是有明显的修复效果。
To better explore the polysaccharides of Oenothera erythrosepala, the optimal extraction process of Oenothera erythrosepala polysaccharides was studied with the response surface method under a single factor. The content of polysaccharides from Oenothera erythrosepala in different periods was determined. Meanwhile, the repairing effect of polysaccharides from Oenothera erythrosepala was evaluated using RAW264.7 cells damaged by hydrogen peroxide. The results showed that the microwave extraction is more effective than the ultrasonic extraction and the traditional extraction. Through the single factor and the response surface analysis, the best extraction conditions of polysaccharide were microwave power 460 w, extraction time 25 min, feed liquid ratio 1:20, and the extraction rate under this condition was 78.55%. The contents of Oenothera erythrosepala po-lysaccharide in the different periods and soil showed that the highest content of polysaccharide was in November and sand. The activity test showed that Oenothera erythrosepala polysaccharide can effectively repair the damage of RAW264.7 by hydrogen peroxide oxidation.

References

[1]	张梅, 刘利. 月见草的开发利用及栽培技术[J]. 农牧产品开发, 1999(10): 28.
[2]	汪开治. 妇科良药月见草[J]. 植物杂志, 2002(6): 14.
[3]	张梅. 月见草的特殊功效及其开发前景[J]. 食品研究与开发, 2006, 27(4): 144-146.
[4]	卢金清, 许家琦, 何冬黎, 等. 月见草全草挥发油成分的气相色谱-质谱联用分析[J]. 中国医院药学杂志, 2011, 31(14): 1225-1226.
[5]	王振斌, 刘加友, 马海乐, 等. 无花果多糖提取工艺优化及其超声波改性[J]. 农业工程学报, 2014, 30(10): 262-269.
[6]	Xu, Z., Li, Y. and Bi, H.T. (2009) Total Fractionation and Charac-terization of the Water-Soluble Polysaccharides Isolated from Panax Ginseng C. A. Meyer. Carbohydrate Polymers, 77, 544-552.
[7]	卫强, 桂芹, 邱镇, 等. 紫荆花中多糖的微波提取工艺优化及其抗氧化活性[J]. 食品科学, 2015, 36(4): 39-44.
[8]	黄晓君, 聂少平, 王玉婷, 等. 铁皮石斛多糖提取工艺优化及其成分分析[J]. 食品科学, 2013, 34(22): 21-26.
[9]	Temelli, F. (2006) Extraction and Functional Properties of Barley β-Glucan as Affected by Tem-perature and pH. Journal of Food Science, 62, 1194-1201.
[10]	刘言炜, 张发宇, 汪家权. 响应面法优化超声波辅助冻融提取蓝藻多糖工艺研究[J]. 食品科技, 2018, 43(5): 198-205.
[11]	唐健波, 肖雄, 杨娟, 等. 响应面优化超声辅助提取刺梨多糖工艺研究[J]. 天然产物研究与开发, 2015, 27(2): 314-320.
[12]	Nyam, K.L., Tan, C.P., Lai, O.M., et al. (2009) Enzyme-Assisted Aqueous Extraction of Kalahari Melon Seed Oil: Optimization Using Response Surface Methodology. Journal of the American Oil Chemists’ Society, 86, 1235-1240. https://doi.org/10.1007/s11746-009-1462-8
[13]	张居作, 许巧玲, 徐君飞. 苦瓜多糖含量的苯酚硫酸法检测研究[J]. 食品研究与开发, 2015, 36(5): 82-85.
[14]	王玉荣, 杨康, 崔伟业, 等. 绞股蓝黄酮对过氧化氢损伤A549细胞的作用[J]. 中国中药杂志, 2018, 43(5): 1014-1020.
[15]	罗磊, 张冰洁, 马丽苹, 等. 金银花叶黄酮体外抗氧化能力及对H2O2诱导RAW264.7巨噬细胞损伤的保护作用[J]. 食品科学, 2018, 39(11): 139-145.
[16]	陈立江, 王永杰, 刘宇, 等. 星点设计-效应面法优选南瓜多糖提取工艺[J]. 食品科学, 2013, 34(8): 107-112.
[17]	王燕华, 武福华, 郭昭涵, 等. 响应面试验优化丹参中多糖的超声波提取工艺及其抗氧化活性[J]. 食品科学, 2015, 36(18): 7-12.
[18]	邓黎, 王晓虹, 韩涛, 等. 响应曲面法优化虫草素和虫草多糖的综合提取工艺[J]. 天然产物研究与开发, 2013, 25(10): 1428-1435+1427.
[19]	Wang, C., Xu, L., Guo, X., Cui, X. and Yang, Y. (2018) Optimization of the Extraction Process of Polysaccharides from Dendrobium Officinale and Evaluation of the in Vivo Immunmodulatory Activity. Journal of Food Processing and Preservation, 42, e13598. https://doi.org/10.1111/jfpp.13598
[20]	Wu, Y., Cui, S., Tang, J. and Gu, X. (2007) Optimization of Extraction Process of Crude Polysaccharides from Boat-Fruited Sterculia Seeds by Response Surface Methodology. Food Chemi-stry, 105, 1599-1605. https://doi.org/10.1016/j.foodchem.2007.03.066
[21]	张晓静, 刘会东. 植物多糖提取分离及药理作用的研究进展[J]. 时珍国医国药, 2003, 14(8): 495-497.
[22]	蒋德旗, 黄利敏, 王艳, 等. 响应面优化纤维素酶法提取桂花多糖工艺及其抗氧活性研究[J]. 食品工业科技, 2015, 36(2): 271-275.
[23]	Liang, R.J. (2008) Optimization of Ex-traction Process of Glycyrrhiza Glabra Polysaccharides by Response Surface Methodology. Carbohydrate Polymers, 74, 858-861. https://doi.org/10.1016/j.carbpol.2008.05.005
[24]	汪瑞敏, 朱秋劲, 张春花, 等. 不同提取方法对天麻多糖抗氧化活性的影响[J]. 食品科技, 2015, 40(3): 208-213.
[25]	李云, 朱彩平, 张扬, 等. 微波辅助提取石榴皮多糖工艺优化及其免疫调节作用研究[J]. 天然产物研究与开发, 2018, 30(6): 1015-1020+1077.
[26]	Jacob, J., Chia, L.H.L. and Boey, F.Y.C. (1995) Review-Thermal and Nonthermal Interaction of Microwave Radiation with Materials. Journal of Materials Science, 30, 5321-5327. https://doi.org/10.1007/BF00351541
[27]	李云, 朱彩平, 张扬, 等. 微波辅助提取石榴皮多糖工艺优化及其免疫调节作用研究[J]. 天然产物研究与开发, 2018, 30(6): 1015-1020+1077.
[28]	Gao, S., Han, W. and Deng, X. (2004) Study of the Mechanism of Microwave-Assisted Ex-traction of Mahonia bealei (Fort.) Leaves and Chrysanthemum Morifolium (Ramat.) Petals. Flavour and Fragrant Journal, 19, 244-250. https://doi.org/10.1002/ffj.1296
[29]	谭丽鹤, 李红玉. 桑蚕蛹多糖超声提取优化及水溶性多糖组分分析[J]. 中成药, 2016, 38(6): 1254-1259.

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