VIGS (Virus-induced gene silencing) can effectively
silence target genes at the RNA level to investigate their functions. The virus
vectors used for silencing are divided into
three categories, DNA virus, RNA virus, and satellite virus, which can be used
to silence the target genes in different species based on existing research.
The genes used as markers for silencing system identification include PDS and
PCNA. VIGS can be applied to some plants, in particular cash crops and
fruit trees, to study their disease-resistance genes and genes related to
growth and development to provide a basis for improving crop characteristics.
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