In order to determine the most suitable
cytokinin for the micropropagation of carob (Ceratonia siliqua L.), the effect of four cytokinins: BAP, zeatin,
kinetin and 2-iP, was tested on explants derived from young seedlings of seven
days. Organogenesis is better in the presence of BAP (0.5 mg/l), while buds
growth is favored by zeatin (0.5 mg/l). The combination of the most suitable
cytokinin (BAP at 0.5 mg/l) with IBA improves the caulogenesis when the
concentration of this latter is low (0.1 mg/l); high concentrations of IBA have
an inhibitory effect on elongation and neoformation of shoots and leaves. The
multiplication and growth of shoots are more favorable on WPM medium in the
presence of BAP (0.5 and 1 mg/l) alone or combined with 0.5 mg/l with GA3,
while rooting is mainly favored by IBA, especially at 2 mg/l.
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