The present study was conducted to investigate the
effect of agitation rate on the increase in fresh weight of MD2 pineapple
protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were
cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and
plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L
1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80,
100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the
highest fresh weight and the highest number of shoots at 76 g and 41 shoots,
respectively. A comparative study of agitation found that 80 rpm was the best
speed which enhanced both PLB and shoot formation. The findings in the present
study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.
References
[1]
Hamid, N.S., Bukhori, M.F.M. and Jalil, M. (2013) Direct and Indirect Plant Regenerations of Pineapple var. MD2 (Ananas comosus L.). Malaysian Applied Biology Journal, 42, 61-66.
[2]
Morton, J.F. (1987) Pineapple, Ananas comosus.
[3]
Coppens d’Eeckenbrugge, G. and Leal, F. (2003) Morphology, Anatomy, and Taxonomy. In: Bartholomew, D.P., Paull, R.E. and Rohrbach, K.G., Eds., The Pineapple: Botany, Production, and Uses, CABI Publishing, Wallingford, 21.
[4]
Birgit, B. (2010) Pineapple Growing. http://tropicalpermaculture.com
Anonymous (2006) Pineapple News. Newsletter of the Pineapple Working Group, International Society for Horticultural Science.
[7]
Malaysian Pineapple Industry Board. http://www.mpib.gov.my
[8]
Danso, K.E., Ayeh, K.O., Oduro, V., Amiteye, S. and Amoatey, H.M. (2008) Effect of 6-Benzylaminopurine and Naphthalene Acetic Acid on in Vitro Production of MD2 Pineapple Planting Materials. World Applied Sciences Journal, 3, 614-619.
[9]
Akbar, M.A., Karmarka, B. and Roy, S. (2003) Callus Induction and High Frequency Plant Regeneration of Pineapple (Ananas comosus L. Merr.). Plant Tissue Culture, 13, 109-116.
[10]
Rahman, Z.A., Abbas, H. and Othman, A.N. (2018) Rapid Micropropagation of MD2 Pineapple (Ananas comosus L.) Using the Temporary Immersion Systems (TIS) and Liquid-Shake Culture (LSC). Asian Journal of Science and Technology, 9, 8833-8836.
[11]
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[12]
Akin-Idowu, P.E., Solomon Akinyemi, S.O. and Olubunmi Ibitoye, D. (2014) Influence of Medium Type and Growth Regulators on in Vitro Micropropagation of Pineapple (Ananas comosus (L.), var. Smooth cayenne). African Journal of Plant Science, 8, 450-456. https://doi.org/10.5897/AJPS2014.1184
[13]
Ziv, M (2005) Simple Bioreactors for Mass Propagation of Plants. In: Hvoslef-Eide, A.K. and Preil, W., Eds., Liquid Culture Systems for in Vitro Plant Propagation, Springer-Verlag, Dordrecht, 79-94. https://doi.org/10.1007/1-4020-3200-5_5
[14]
Vyas, S., Rao, M.S., Suthar, R.K. and Purohit, S.D. (2008) Liquid Culture System Stimulates in Vitro Growth and Shoot Multiplication in Four Medicinally Important Plants. Medicinal and Aromatic Plant Science and Biotechnology, 2, 96-100.
[15]
Etienne, H. and Berthouly, M. (2002) Temporary Immersion Systems in Plant Micropropagation. Plant Cell, Tissue and Organ Culture, 69, 215-231.
https://doi.org/10.1023/A:1015668610465
