本研究依托ASE-online SPE-HPLC/MS快速筛选平台,建立了基于谱效关系快速筛选康定鼠尾草中抗氧化活性成分的方法,并筛选出5个具有抗氧化活性的化合物,通过质谱数据鉴别为丹参素、原儿茶醛、咖啡酸、异迷迭香酸苷和迷迭香酸。针对实验过程中的快速溶剂萃取条件、在线固相萃取条件以及质谱检测条件进行了优化。该方法将活性测试与质谱鉴别相结合,具有前处理高效、操作简单、重现性良好等优势,适合从复杂基质的中藏药材中快速筛选并鉴别抗氧化活性成分,大大提高了效率。
In this study, based on the rapid screening platform of ASE-online SPE-HPLC/MS, a rapid screening method based on chromatography-efficacy relationship was established to screen antioxidants from Salvia prattii. Five compounds with antioxidant activity were screened, which were identified as tanshinol, protocatechualdehyde, caffeic acid, salviaflaside and rosmarinic acid by mass spectrometry data. The conditions of ASE, on-line SPE and MS were optimized. This method com-bines the activity test with mass spectrometry identification, and has the advantages of high pre-treatment efficiency, simple operation and good reproducibility. It is suitable for the rapid screening and identification of antioxidant active components from medicinal plants with complex substrates, and meanwhile greatly improving the efficiency.