将废弃角蛋白水解洗涤过滤制得高纯复合氨基酸，利用水溶液合成法制备复合氨基酸螯合锌，用EDTA滴定法和原子吸收分光光度法分别测定复合氨基酸螯合锌的螯合率，其相对标准偏差分别为2.79%和1.38%。以甲硫氨酸和丙氨酸为例，分别对甲硫氨酸螯合锌和丙氨酸螯合锌进行红外光谱表征，其吸收峰明显减少，说明产物的对称性增加，结合吸收峰的位移变化说明了螯合物的生成。
The high-purity complex amino acid was prepared by washing and filtering the waste keratin, and the corresponding complex chelated amino acidic zinc compounds were prepared by aqueous so-lution synthesis method. The chelated rate of the complex chelated amino acidic zinc compound was determined by EDTA titration and atomic absorption spectrophotometry, respectively. The standard deviations were 2.79% and 1.38%. Taking methionine and alanine as examples, the spectroscopy of zinc methionine and alanine chelate zinc were characterized by infrared spec-troscopy and the amount of the absorption peak was significantly reduced. The change of the dis-placement of the absorption peak indicates the formation of the complex chelatedamino acidic zinc.
References
[1]
张瑞强. 锌的营养学研究进展[J]. 家畜生态学报, 2003, 24(4): 66-68.
[2]
Sun, Y., Mi, W. and Wu, L. (2018) Effects of Foliar Fe and Zn Fertilizers on Storage Root Fe, Zn, and Beta-Carotene Content of Sweet Potato (Ipomoea batatas L.). Journal of Plant Nutrition, 42, 16-26.
https://doi.org/10.1080/01904167.2018.1510505
Liu, H., Zhang, R., Yao, X., et al. (2004) Prediction of the Isoelectric Point of an Amino Acid Based on GA-PLS and SVMs. Journal of Chemical Information and Modeling, 44, 161-167. https://doi.org/10.1021/ci034173u
[7]
Ghasemi, S., Khoshgoftarmanesh, A.H., Afyuni, M. and Hadadzadeh, H. (2013) The Effectiveness of Foliar Applications of Synthesized Zinc-Amino Acid Chelates in Comparison with Zinc Sulfate to Increase Yield and Grain Nutritional Quality of Wheat. European Journal of Agronomy, 45, 68-74.
