To screen and characterize
the analgesic components from Marasmius
androsaceus, the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol
precipitation followed by macroporous resin chromatography and activity-based
fractionation. Analgesic activities were found to be associated with
glycopeptides. The glycopeptide fractions were further purified by gel-permeation
chromatography and two purified glycopeptides, D2H, D2L, were obtained. Next,
the physicochemical properties and characteristics of these purified
glycopeptides were studied. They were composed of glucose and mannose and 10
different amino acids and with molecular weights of 18,000 and 8000 Da,
respectively. The glycosidic linkages in the purified glycopeptides were
analysed via methylation analysis. The sugar portion of the glycopeptides was
composed of 1-4 linked Glc as the main chain with side chains
composed primarily of Man linked to the 6 position of the main chain; the
non-reducing terminal was composed of Glc. The glycosidic band in the main
chain was identified as being in the β-configuration
by the IR absorbance at 891 cm-1.
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